Pojen Shih

6376248, Apr 23, 2002

Mar 16, 1998

09/039780

Jianqing Lan - Germantown MD
PoJen Shih - Columbia MD
Joel A. Jessee - Mt. Airy MD
Kevin P. Schifferli - Germantown MD
Gulilat Gebeyehu - Silver Spring MD
Valentina C. Ciccarone - Gaithersburg MD
Krista L. Evans - Germantown MD

Life Technologies, Inc. - Rockville MD

C12N 1588

435458, 4353201, 4352351, 536 231

The present invention provides compositions useful for transfecting eukaryotic cells comprising nucleic acid complexes with peptides, wherein the peptide is optionally covalently coupled to a nucleic acid-binding group, and cationic lipids or dendrimers as transfection agents. The invention also provides transfection compositions in which a peptide is covalently linked to the transfection agent (lipid, cationic lipid or dendrimer). Inclusion of peptides or modified-peptides in transfection compositions or covalent attachment of peptides to transfection agents results in enhanced transfection efficiency. Methods for the preparation of transfection compositions and methods of using these transfection compositions as intracellular delivery agents and extracellular targeting agents are also disclosed.

8058068, Nov 15, 2011

Jul 23, 2002

10/200879

Jianqing Lan - Germantown MD, US
PoJen Shih - Columbia MD, US
Joel A. Jessee - Mt. Airy MD, US
Kevin P. Schifferli - Germantown MD, US
Gulilat Gebeyehu - Silver Springs MD, US
Valentina C. Ciccarone - Gaithersburg MD, US
Krista L. Evans - Germantown MD, US

Life Technologies Corporation - Carlsbad CA

C12N 15/88
C07K 14/00
C07K 14/025
C07H 21/02
C07H 21/04

435458, 530324, 4284022, 536 231

The present invention provides compositions useful for transfecting eukaryotic cells comprising nucleic acid complexes with peptides, wherein the peptide is optionally covalently coupled to a nucleic acid-binding group, and cationic lipids or dendrimers as transfection agents. The invention also provides transfection compositions in which a peptide is covalently linked to the transfection agent (lipid, cationic lipid or dendrimer). Inclusion of peptides or modified-peptides in transfection compositions or covalent attachment of peptides to transfection agents results in enhanced transfection efficiency. Methods for the preparation of transfection compositions and methods of using these transfection compositions as intracellular delivery agents and extracellular targeting agents are also disclosed.

20030069173, Apr 10, 2003

Jul 23, 2001

09/911569

Jianqing Lan - Germantown MD, US
PoJen Shih - Columbia MD, US
Joel Jessee - Mt. Airy MD, US
Kevin Schifferli - Germantown MD, US
Gulilat Gebeyehu - Silver Spring MD, US
Valentina Ciccarone - Gaithersburg MD, US
Krista Evans - Germantown MD, US

Life Technologies, Inc.

A61K048/00
C12N015/88

514/008000, 514/044000, 435/458000

The present invention provides compositions useful for transfecting eukaryotic cells comprising nucleic acid complexes with peptides, wherein the peptide is optionally covalently coupled to a nucleic acid-binding group, and cationic lipids or dendrimers as transfection agents. The invention also provides transfection compositions in which a peptide is covalently linked to the transfection agent (lipid, cationic lipid or dendrimer). Inclusion of peptides or modified-peptides in transfection compositions or covalent attachment of peptides to transfection agents results in enhanced transfection efficiency. Methods for the preparation of transfection compositions and methods of using these transfection compositions as intracellular delivery agents and extracellular targeting agents are also disclosed.

20060229246, Oct 12, 2006

Jan 17, 2006

11/333982

Jianqing Lan - Germantown MD, US
PoJen Shih - Columbia MD, US
Joel Jessee - Mt. Airy MD, US
Keivn Schifferli - Germantown MD, US
Gulilat Gebeyehu - Silver Spring MD, US
Valentina Ciccarone - Gaithersburg MD, US
Krista Evans - Germantown MD, US

A61K 38/17
A61K 48/00
C12N 15/88
C12N 15/86
C12Q 1/68

514012000, 435456000, 435455000, 514044000, 435006000, 435458000

The present invention provides compositions useful for transfecting eukaryotic cells comprising nucleic acid complexes with peptides, wherein the peptide is optionally covalently coupled to a nucleic acid-binding group, and cationic lipids or dendrimers as transfection agents. The invention also provides transfection compositions in which a peptide is covalently linked to the transfection agent (lipid, cationic lipid or dendrimer). Inclusion of peptides or modified-peptides in transfection compositions or covalent attachment of peptides to transfection agents results in enhanced transfection efficiency. Methods for the preparation of transfection compositions and methods of using these transfection compositions as intracellular delivery agents and extracellular targeting agents are also disclosed.

56271590, May 6, 1997

Oct 27, 1994

8/329969

PoJen Shih - Columbia MD
Joel A. Jessee - Mt. Airy MD

Life Technologies, Inc. - Gaithersburg MD

A61K 4800
A61K 9127
C12N 500

514 44

A method is provided for enhancing transfection efficiency of eukaryotic cells comprising contacting said cells with a lipid aggregate comprising nucleic acid and a polycationic lipid composition in the presence of a polycationic compound and serum. The polycationic compound is preferably Polybrene. TM. , and the lipid aggregate preferably comprises liposomes of nucleic acid and LipofectAMINE. TM.

57363927, Apr 7, 1998

Jun 4, 1996

8/658130

Jianqing Lan - Germantown MD
PoJen Shih - Columbia MD
Joel A. Jessee - Mt. Airy MD
Kevin P. Schifferli - Germantown MD

Life Technologies, Inc. - Rockville MD

C12N 1500
C07K 14005
G01N 3392

4353201

The present invention discloses compositions useful for transfecting eukaryotic cells comprising nucleic acid complexes with peptides, proteins or protein fragments, wherein the peptide is optionally covalently coupled to a DNA-binding group, and cationic lipids useful for transfecting eukaryotic cells. Methods for the preparation of transfecting compositions and use as intracellular delivery agents and extracellular targeting agents are also disclosed.

60514299, Apr 18, 2000

Mar 14, 1997

8/818200

Jianqing Lan - Germantown MD
PoJen Shih - Columbia MD
Joel A. Jessee - Mt. Airy MD
Kevin P. Schifferli - Germantown MD
Gulilat Gebeyehu - Silver Spring MD

Life Technologies, Inc. - Rockville MD

C12N 1564
C12N 1563
C12N 700
C12N 1511

435458

The present invention provides compositions useful for transfecting eukaryotic cells comprising nucleic acid complexes with peptides, wherein the peptide is optionally covalently coupled to a nucleic acid-binding group, and cationic lipids or dendrimers as transfection agents. The invention also provides transfection compositions in which a peptide is covalently linked to the transfection agent (lipid, cationic lipid or dendrimer). Inclusion of peptides or modified-peptides in transfection compositions or covalent attachment of peptides to transfection agents results in enhanced transfection efficiency. Methods for the preparation of transfection compositions and methods of using these transfection compositions as intracellular delivery agents and extracellular targeting agents are also disclosed.