Ming Xu

20050100987, May 12, 2005

Oct 7, 2004

10/960905

Thomas Evans - Somerville MA, US
Ming Xu - Hamilton MA, US
Shaorong Chong - Somerville MA, US

C12P021/06
C07H021/04
C12N009/00
C12N001/18
C12N005/06
C12N015/74

435069100, 435320100, 435471000, 435325000, 435348000, 435254200, 435252100, 435183000, 530350000, 536023200

A method for the ligation of expressed proteins which utilizes inteins, for example the RIR1 intein from , is provided. Constructs of the Mth RIR1 intein in which either the C-terminal asparagine or N-terminal cysteine of the intein are replaced with alanine enable the facile isolation of a protein with a specified N-terminal, for example, cysteine for use in the fusion of two or more expressed proteins. The method involves the steps of generating a C-terminal thioester-tagged target protein and a second target protein having a specified N-terminal via inteins, such as the modified Mth RIR1 intein, and ligating these proteins. A similar method for producing a cyclic or polymerized protein is provided. Modified inteins engineered to cleave at their C-terminus or N-terminus, respectively, and DNA and plasmids encoding these modified inteins are also provided.

20220347876, Nov 3, 2022

May 3, 2021

17/306685

- Boston MA, US
Ming Laura Xu - Natick MA, US

B26B 21/60
B05D 1/02

Razor blades with two or more materials and/or methods of fabricating the same. In one example, a bimetal razor blade can comprise a blade body and a blade edge. The blade body can have a length and a width. The blade body can be formed of a first substrate portion comprising a first material having a first hardness. The blade edge can extend along the length. The blade edge can be formed of a second substrate portion coupled to the first substrate portion. The second substrate portion can comprise a second material having a second hardness that is distinct from the first hardness. The first and second materials can be metals.

20210283185, Sep 16, 2021

Jul 3, 2019

17/257923

- Rochester MN, US
- Boston MA, US
Stefan Tullius - Boston MA, US
Ming Xu - Avon CT, US
Joao Passos - Rochester MN, US

A61K 35/28
A61K 31/506
A61K 31/353
A01K 67/027
A61P 39/00

This document provides methods and materials for treating aging and/or improving transplant outcomes. For example, methods and materials for using one or more senotherapeutic agents to reduce risk of transplant rejection are provided. Non-human animal models for transplant rejection as well as methods for using such non-human animal models to identify agents having the ability to reduce transplant rejection are provided as are non-human animal models for aging and methods for using such non-human animal models to identify agents having the ability to treat aging or the ability to slow the effects of aging.

20180126574, May 10, 2018

Oct 16, 2017

15/784453

- Boston MA, US
Ming Laura Xu - Natick MA, US
John Lawrence Maziarz - Framingham MA, US

B26B 21/56
B26B 21/22
B26B 21/06
B26B 21/40

A method of manufacturing a razor blade by providing a razor blank strip having an edge. The razor blank strip is hardened to a hardness greater than 600HV. A pre-bending zone that is spaced apart from an edge of the razor blank is melted with a laser beam. The pre-bending zone is cooled after melting to form a re-solidification portion. The razor blank strip is segmented into individual blades. The individual blades are bent in the pre-bending zone.

20170136640, May 18, 2017

Nov 13, 2015

14/940858

- Boston MA, US
Ming Laura Xu - Natick MA, US
Yongqing Ju - Medfield MA, US
Bin Shen - Allston MA, US
Matthew Robert Stone - Oxford MA, US

B26B 21/60
B26B 21/56

A razor blade having a substrate with a cutting edge being defined by a sharpened tip is provided where the substrate is comprised of a molybdenum (Mo) content of about 1.6% to about 5% by weight of composition and thicknesses of 1.60-1.75 μm and 9.25-10.00 μm measured at a distance of 4 and 40 μm from the blade tip, respectively. This thickness ratio is between 0.165-0.185. The substrate thickness is about 2.70-3.00 μm at 8 μm from the blade tip, about 4.44-5.00 μm at 16 μm from the blade tip with a thickness ratio measured at 4 μm and 8 μm between 0.56-0.62, and a thickness ratio measured at 4 μm and 16 μm between 0.32-0.40. The edge shape may be defined by equation w=adwith “a” between 0.50-0.62 and “n” between 0.76-0.80. A semi-included angle less than 7 is measured at a distance of 40 μm or greater from the blade tip. This substrate will have substantially no cracks.