Resumes
Resumes
Matthew Posewitz Phones & Addresses
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224 Bannock St, Denver, CO 80223
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Golden, CO
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Florissant, CO
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Helena, MT
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900 190Th St, New York, NY 10040
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Salt Lake City, UT
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Golden, CO
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Jefferson, CO
Publications
Us Patents
In Vivo Evolution Of Hydrogenases Using A Hydrogen-Sensing System
View pageUS Patent:
20110059863, Mar 10, 2011
Filed:
Mar 10, 2009
Appl. No.:
12/918182
Inventors:
Maria Lucia Ghirardi - Lakewoood CO, US
Matt Wecker - Boulder CO, US
Matthew Posewitz - Golden CO, US
Matt Wecker - Boulder CO, US
Matthew Posewitz - Golden CO, US
Assignee:
Alliance for Sustainable Energy, LLC - Golden CO
International Classification:
C40B 30/08
C12Q 1/26
C12N 15/63
C12N 1/00
C12Q 1/26
C12N 15/63
C12N 1/00
US Classification:
506 11, 435 25, 4353201, 435243
Abstract:
Provided herein are methods for measuring H2 production by a hydrogenase. Also provided are methods for evolving a hydrogenase or hydrogen-sensing system by comparing the level of production of H2 by the hydrogenase to the level Of H2 produced by a wild-type hydrogenase cultured under the same conditions and selecting a microorganism with increased H2 production over the H2 production by a microorganism having a wild-type hydrogenase. Further provided herein are microorganisms and plasmids comprising a hupSL promoter coupled with a reporter gene.
Modified Algae For Improved Biofuel Productivity
View pageUS Patent:
20120329099, Dec 27, 2012
Filed:
Mar 26, 2012
Appl. No.:
13/430383
Inventors:
Matthew C. Posewitz - Golden CO, US
Randor Radakovits - Denver CO, US
Robert Jinkerson - Golden CO, US
Victoria H. Work - Golden CO, US
Jonathan E. Meuser - La Jolla CA, US
Randor Radakovits - Denver CO, US
Robert Jinkerson - Golden CO, US
Victoria H. Work - Golden CO, US
Jonathan E. Meuser - La Jolla CA, US
Assignee:
Colorado School of Mines - Golden CO
International Classification:
C12N 1/13
C12P 19/14
C12P 19/14
US Classification:
435 99, 4352572
Abstract:
Methods, processes, and systems for the production of lipids and starch from modified algae are disclosed. In one embodiment, the modified algae over-expresses isoamylase and accumulates much higher amounts of starch than unmodified algae. In some embodiments, the modified algae comprises one or more copies of an isoamylase expression construct. In one embodiment, the modified algae is a sta7 mutant with a starchless phenotype that has been complemented with one or more copies of the wild-type genomic STA7 isoamylase gene construct. The complemented, modified algae accumulates much greater amount of starch than an unmodified algae and may be used to produce large amounts of starch and/or lipids.
Use Of Endogenous Promoters In Genetic Engineering Of Nannochloropsis Gaditana
View pageUS Patent:
20130102040, Apr 25, 2013
Filed:
Oct 17, 2012
Appl. No.:
13/654347
Inventors:
Randor Radakovits - Denver CO, US
Robert Jinkerson - Golden CO, US
Matthew Posewitz - Golden CO, US
Robert Jinkerson - Golden CO, US
Matthew Posewitz - Golden CO, US
Assignee:
COLORADO SCHOOL OF MINES - Golden CO
International Classification:
C12N 15/79
US Classification:
435134, 4353201, 4352572, 435471, 536 241, 536 237
Abstract:
The present disclosure is directed to novel polynucleotide sequences for use in . The novel polynucleotide sequences include control sequences and coding sequences. Also disclosed are novel gene expression constructs wherein promoters/control regions are operatively linked to or non-coding sequences. These novel polynucleotide sequences and expression constructs can be introduced into and can recombine into the genome. Expression from these polynucleotide sequences and expression constructs can enhance biomass and/or lipid biosynthesis. Also disclosed are methods for modifying , for example by stably transforming with nucleic acid sequences, growing the modified , and obtaining biomass and biofuels from the modified
Process And Genes For Expression And Overexpression Of Active [Fefe] Hydrogenases
View pageUS Patent:
20090087880, Apr 2, 2009
Filed:
Feb 28, 2006
Appl. No.:
11/573035
Inventors:
Michael Seibert - Lakewood CO, US
Paul W. King - Golden CO, US
Maria Lucia Ghirardi - Lakewood CO, US
Matthew C. Posewitz - Golden CO, US
Sharon L. Smolinski - Littleton OH, US
Paul W. King - Golden CO, US
Maria Lucia Ghirardi - Lakewood CO, US
Matthew C. Posewitz - Golden CO, US
Sharon L. Smolinski - Littleton OH, US
International Classification:
C12P 21/04
C12N 1/21
C12N 1/21
US Classification:
435 691, 43525233
Abstract:
A process for expression of active [FeFe]-hydrogenase in a host organism that does not contain either the structural gene(s) for [FeFe]-hydrogenases and/or homologues for the maturation genes HydE, HydF and HyG, comprising: cloning the structural hydrogenase gene(s) and/or the maturation genes HydE, HydF and HydG from an organisms that contains these genes into expression plasmids; transferring the plasmids into an organism that lacks a native [FeFe]-hydrogenase or that has a disrupted [FeFe]-hydrogenase and culturing it aerobically; and inducing anaerobiosis to provide [FeFe] hydrogenase biosynthesis and H?2#191 production.