Inventors:
Ronald W. Davis - Palo Alto CA
Guri N. Giaever - Palo Alto CA
Assignee:
The Board of Trustees of the Leland Stanford Junior University - Palo Alto CA
International Classification:
C12Q 168
US Classification:
435 6, 435 71, 435 72, 435 29, 4352523, 4352542, 435455
Abstract:
The invention features methods of high throughput screening of candidate drug agents and rapid identification of drug targets by examining induction of the stress response in a host cell, e. g. , the stress response in wildtype host cells and/or in host cells that differ in target gene product dosage (e. g. , host cells that have two copies of a drug target gene product-encoding sequence relative to one copy). In general, induction of the stress response in wildtype host cells indicates that a candidate agent has activity of the drug. Induction of a relatively lower or undetectable stress response in a host cell comprising an alteration in gene product dosage indicates that the host cell is drug-sensitive and is altered in a gene product that plays a role in resistance to the drug.