Brit J Hart

Washington, DC

Non-Profit Organization Management

Aphl - Association of Public Health Laboratories Nov 2015 - Jun 2017
Manager, Biosafety and Biosecurity
Naval Medical Research Center Biological Defense Research Directorate Nov 2015 - Jun 2017
Administrator, Biosurety Program and Responsible Official
National Institute of Allergy and Infectious Diseases (Niaid) Mar 2009 - Nov 2015
Scientist
United States Marine Corps Oct 1998 - Oct 2004
Combat Engineer

The Johns Hopkins University 2000 - 2002
Masters, Biotechnology William & Mary 1994 - 1998
Bachelors, Psychology

Molecular Virology
Risk Tolerance
Military Leadership
Virology
Savings
Biomedical Engineering
Biotechnology
Infectious Diseases
Time Value of Money
Polymerase Chain Reaction
Elisa
Molecular Cloning
Real Time Polymerase Chain Reaction
In Vivo
Dna

Shipping Certification: Certified For the Shipping of Infectious Substances, Diagnostic Specimens, Biological Specimens and Dry Ice Classified As "Dangerous Goods."​
Approval For Access To Select Agent(S) and/Or Toxins (Us Department of Justice)-Inactive
Us Access National Agency Check and Inquiries (Anaci) Clearance

NIH / NIAID / INTEGRATED RESEARCH FACILITY

Mar 2009 to 2000
Support Scientist
VIRxSYS CORPORATION, R&D Department
Gaithersburg, MD
May 2006 to Feb 2009
Research Associate IV
FUNCTIONAL GENETICS, R&D Department Rockville, MD. October 2001 April 2006
Rockville, MD
Oct 2001 to Apr 2006
Research Associate II
LIFE TECHNOLOGIES / INVITROGEN, R&D Department
Rockville, MD
Jan 2001 to Sep 2001
Associate Scientist II
CELERA GENOMICS, Pre-Sequencing Department
Rockville, MD
Jun 1999 to Jan 2001
Lab Technician II

JOHNS HOPKINS UNIVERSITY
Baltimore, MD
2000 to 2002
M.S. in Biotechnology
College of William and Mary
Williamsburg, VA
1994 to 1998
B.S. in Biological Psychology, Minor in Biochemistry

Rank: Cpl. Jul 1998 to Jul 2004
Branch: UNITED STATES MARINE CORPS RESERVES,L.i.location.original

DNA: DNA purification, Advanced Molecular Cloning, Restriction Enzyme Digestion Analysis, Polymerase Chain Reaction (PCR), Nucleic Acid Sequencing, Southern Blot, Bacterial Cell Transformation, Oligonucleotide design, Site-Directed Mutagenesis, Quantitative Real Time PCR, Transposon Library Construction, Spectrophotometric and Fluorometric Analysis. RNA: RNA isolation and purification, Reverse Transcriptase PCR, Quantitative RT PCR, Northern Blot, si-RNA, RNA Protect Assays, cDNA library construction. Protein / Antibody: SDS-PAGE, Western Blot, Immunoprecipitation, ELISA Assay, Immunofluorescence Assay , High Performance Liquid Chromatography (HPLC); Size-exclusion, Ion exchange, Affinity Chromatography, Large Scale Antibody Production/Purification, Antibody Conjugation/Labeling, Fab Production, Protein Extraction, Antibody Isotype Assay, Lowry Protein Assay, Bradford Protein Assay, High Content-Image Analysis, Hybridoma Antibody Production. Tissue / Cell: Aseptic Tissue Culture Technique, Transfection, Transduction, Flow Cytometry/ Fluorescence-Activated Cell Sorting (FACS), Reporter Gene Assays, Confocal and Fluorescent Microscopy, Cryopreservation, Cell fixing and staining, Hybridoma Culture, Subcloning, Serum Free Adaption, Large-Scale Multi-layer Flasks/Roller Bottle Culture. Experience with MDCK, CHO, 293T, HEK293, SupTI, Hep2, Jurkat, HeLa, BSC-1, HCT-116, Cos-1, VeroE6, MRC-5, various Primary Cell-Lines. Virus / Vector: Viral Infection, Virus Production and Purification, Recombinant Vector Construction/Production, Virus Titration, Plaque Assay, TCID50 Assay, Hemagglutination Assay, Viral Neutralization Assay, gradient making & centrifugation. Experience with Ebola, HIV-1, Influenza, Adenovirus, Vaccinia Virus, Rift Valley Fever Virus, West Nile Virus, Middle Eastern Respiratory Syndrome Virus, Recombinant Viruses and Lentiviral Vectors. Assay Development: Developed; Small Molecule Drug Screen, Anti-Viral Functional Screening Assay, PCR based Assays, ELISA based Assays, Competition Assays, Saturation Assays, Cell Viability Assays, Quantitative Real Time PCR based assays (DNA and RNA), flow cytometry based assays. BSL-3 / BSL4: Trained and experienced in working in a BSL-3 high containment and BSL-4 maximum containment laboratories with over 100 entries in the BSL-4. Radiation: Trained and experienced in working with radioactive materials, such as 32P & 33P labeling. Animal Experiments: Trained and experienced with small animal handling, injections (IV, IM, IP), anesthesia, In Vivo Imaging. Experience with rodents, i.e. Sprague Dawley Laboratory Rats, Balb-C, nude mice, etc. Automation / Robotics: Experience with all types of automation and robotics, including programming; Tecan Freedom EVO Multi Assay Workstation, Beckman Coulter SPAN 8, QIAcube, Biorobot EZ1 workstation, Biotec, Tomtec, Titertek, Q-bot, Q-pick. Shipping Certification: Certified for the shipping of infectious substances, diagnostic specimens, biological specimens and dry ice classified as "dangerous goods." Computer Skills / Software: Microsoft Office (Word, Excel, PowerPoint, Access), Adobe (Acrobat, Photoshop), Lasergene 8 Suite, CloneManager, GelDoc, Magellan, GeneSnap, GeneSys, EvoWare Plus, Primer Express, GraphPad Prism, Flow Jo, Unicorn, BLAST algorithms, Bioinformatics from online sources. Communication Skills: Training and Supervising personnel, Formal Presentations, Technical Writing (Development Reports, SOPs, Scientific Papers), Oral Presentation, Negotiation.