Nan Li

D684703, Jun 18, 2013

Nov 28, 2011

29/407358

Manfred Watzele - Weilheim, DE
Alexander Seiler - Habach, DE
Donald Thomas - Vista CA, US
Wei Li - Hangzhou, CN
Tianxing Wang - Hangzhou, CN
Nan Li - San Diego CA, US
Xiaobo Wang - San Diego CA, US

ACEA Biosciences, Inc. - San Diego CA
Roche Diagnostics Operations, Inc. - Indiana IN

2401

D24224

20110039294, Feb 17, 2011

May 5, 2010

12/774709

Xiaobo Wang - San Diego CA, US
Wei Ouyang - San Diego CA, US
Nan Li - San Diego CA, US
Biao Xi - San Diego CA, US
Yama A. Abassi - San Diego CA, US
Xiao Xu - San Diego CA, US

C12Q 1/02
C12M 1/34

435 29, 4352871

Devices and methods for performing extracellular recording of cells, such as excitable cells, cardiomyocytes, and cardiomyocyte precursor cells is provided. An exemplary device includes a nonconductive substrate forming or provided as a base of one or more wells; a recording electrode positioned on the substrate within the well, wherein the recording electrode is accessible to cells when a cell sample is added to the device; and a reference electrode positioned within the well in a cell-free zone, the cell-free zone characterized as free from contact with cells when the cell sample is added to the device, thereby preventing contact between cells and the reference electrode.

20110300569, Dec 8, 2011

May 17, 2011

13/109809

Nan Li - San Diego CA, US
Xiaobo Wang - San Diego CA, US
Yama A. Abassi - San Diego CA, US
Biao Xi - San Diego CA, US
Wen Fu Zhang - San Diego CA, US
Xiao Xu - San Diego CA, US

ACEA BIOSCIENCES, INC. - San Diego CA

C12Q 1/02

435 29

A method of determining one or more beating parameters for use in cardiomyocyte beating analysis including: providing a cell analysis device including wells, each well including a sensor capable of monitoring beating of cardiomyoctes in millisecond time resolution; adding cardiomyocytes to the wells; monitoring the beating of the cardiomyocytes in millisecond time resolution to obtain a plurality of beating measurements; and calculating one or more beating parameters from the plurality of beating measurements.

20130143254, Jun 6, 2013

Dec 1, 2011

13/309209

Donald Thomas - San Diego CA, US
Wei Li - Hangzhou City, CN
Ning Ke - San Diego CA, US
Nan Li - San Diego CA, US
Adrian Papas - San Diego CA, US
Manfred Watzele - Weilheim, DE
Alexander Seiler - Habach, DE
Tianxing Wang - Hangzhou City, CN
Yama Abassi - San Diego CA, US
Xiaobo Wang - San Diego CA, US

ROCHE DIAGNOSTICS OPERATIONS, INC. - Indianapolis IN
ACEA BIOSCIENCES, INC. - San Diego CA

G01N 27/02
G01N 21/00
C12M 1/34
C12Q 1/02
C12M 1/12
C12M 1/00

435 29, 4352971, 4353052, 4352975, 4352885

An insert device and co-culture device assembly incorporating the insert device, which includes an insert chamber with two fluid impermeable side walls that extend from a microporous bottom to an open top to form an inner cavity. The first side wall is shaped to form a convex arc that follows between about half of a circumference of the well and less than an entire circumference of the well. The second side wall joins ends of the first side wall and protrudes inward towards a center of the convex arc. A flange extends outward from or beneath the top and is notched to form a gap adjacent to the second side wall, which forms an access port allowing access to the lower chamber with a micropipette tip when the insert device is inserted into a well of a single or multi-well plate.

20210041418, Feb 11, 2021

Oct 29, 2020

17/083589

- Santa Clara CA, US
Yama A. Abassi - San Diego CA, US
Nan Li - San Diego CA, US
Lincoln Muir - San Diego CA, US
Xiao Xu - San Diego CA, US

G01N 33/50
G01N 33/543
G01N 27/02

Methods of assessing cytolysis of cancer cells are provided, which include: providing a cell-substrate impedance monitoring device operably connected to an impedance analyzer, adding target cells characterized as cancer cells to a well of the device adding effector cells to the well to form a test well, where the effector cells are immune cells obtained or derived from a same patient as the target cells; monitoring cell-substrate impedance of the test well before and after adding the effector cells and optionally deriving an impedance-based parameter from the impedance; and determining effectiveness of effector cell killing of the target cells by comparing the impedance or impedance-based curves over time.

20200278339, Sep 3, 2020

Jan 24, 2020

16/752298

- San Diego CA, US
Wei Ouyang - San Diego CA, US
Nan Li - San Diego CA, US
Biao Xi - San Diego CA, US
Yama A. Abassi - San Diego CA, US
Xiao Xu - San Diego CA, US

G01N 33/487

Extracellular recording devices with reference electrodes positioned in a cell free zone and methods of conducting extracellular recording. Parallel cell-substrate impedance monitoring and extracellular recording of excitable cells such as excitable cells, cardiomyocytes, and cardiomyocyte precursor cells using devices that switch between extracellular recording and impedance monitoring modes.

20200158670, May 21, 2020

Dec 19, 2019

16/721742

- San Diego CA, US
Wei Ouyang - San Diego CA, US
Nan Li - San Diego CA, US
Tianxing Wang - Hangzhou, CN
Xiaoyu Zhang - San Diego CA, US
Xiao Xu - San Diego CA, US
Yama A. Abassi - San Diego CA, US

G01N 27/02
G01N 33/483

Devices, systems and methods for monitoring excitable cells, such as cardiomyocytes, on microelectrode arrays that couple the electro-stimulation of excitable cells to induce or regulate cardiomyocyte beating and the simultaneous measurement of impedance and extracellular recording to assess changes in cardiomyocyte beating, viability, morphology or electrophysical properties in response to a plurality of treatments.

20200071672, Mar 5, 2020

Mar 3, 2018

16/490302

- San Diego CA, US
Yama A Abassi - San Diego CA, US
Nan Li - San Diego CA, US
Xiaobo Wang - San Diego CA, US

C12N 5/077
C12N 13/00
G01N 27/02
C12N 5/074
C12N 5/00

Methods of inducing functional maturation of immature cardiomyocytes derived from induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs) by electrically pacing the immature cardiomyocytes according to a pulse profile that induces maturation until the immature cardiomyocytes mature into functionally adult cardiomyocytes.