US Patent:
20190083545, Mar 21, 2019
Inventors:
- Stanford CA, US
Dan Xu - Fremont CA, US
International Classification:
A61K 35/407
C12N 5/074
C12N 5/071
Abstract:
Methods are provided for producing a population of hepatocyte-like cells (iHeps) from a population of adipocyte-derived stem cells (ASCs). Aspects of the methods include placing a population of ASCs into a three dimensional culture (e.g., hanging drop suspension culture, high density culture, spinner flask culture, microcarrier culture, etc.), and contacting the cells with a first and second culture medium. Also provided are methods of treating an individual, which include producing a population of iHeps from a population of ASCs, and administering an effective number of iHeps into the individual. Kits for practicing the methods are also described herein.