US Patent:
20160090630, Mar 31, 2016
Inventors:
- Menlo Park CA, US
Jue LIN - Foster City CA, US
Yajing HU - San Jose CA, US
International Classification:
C12Q 1/68
Abstract:
This invention provides methods and materials for measuring telomere abundance from chromosomes in a sample having telomeres within a pre-determined length range, e.g., short telomeres up to a certain length. The methods can involve a first step of performing a time-limited extension reaction calibrated to produce extension products from a double-stranded chromosomal DNA template of no more than a defined length, and a second step of amplifying, from the extension products, sequences bounded by a sub-telomeric sequence and the anchor sequence, to produce a length-limited telomere sequence product. The abundance of telomeric sequences in this product can be measured, and the measures can be correlated to a variety of indices.
