William P Macconnell

20080047835, Feb 28, 2008

Aug 22, 2006

11/466398

William P. MacConnell - Encinitas CA, US

C07K 1/26
G01N 27/00

204456, 204606

A system and method for purifying genomic DNA requires the use of a cassette that is formed with a plurality of wells. Each well has first and second apertures that are respectively covered by a section made of an electrophoretic filter medium (e.g. agarose). In use, a lysate is loaded into the well while the cassette is submerged in a buffer fluid. A voltage cycle is then applied to alternate between forward and reverse electrophoresis in the well, to separate impurities from the lysate for purification of genomic DNA.

47879637, Nov 29, 1988

May 4, 1987

7/046652

William P. MacConnell - Cardiff CA

Syntro Corporation - San Diego CA

C12N 1500

2041801

Method and means for accelerating the rate of hybridization of nucleic acid probes with complemental target sequences in probe assays of the filter binding or sandwich filter binding formats. The nucleic acid probes are electrophoretically concentrated at membrane means including at least a dialysis type membrane and to which the target sequences are bound. The rate of hybridization can be further enhanced by means for moving concentrated unannealed probe molecules successively in various directions along the surface of the membrane means to which the target sequences are bound. The invention further includes method and means for electrophoretically removing from the membrane means unannealed probe molecules which became adsorbed to the membrane means during hybridization. In one embodiment of the invention the membrane means comprises a cellulose dialysis membrane in laminate relation with a nitrocellulose or nylon filter having target sequence molecules bound to the surface thereof facing the dialysis membrane. In another embodiment of the invention the membrane means comprises a dialysis membrane to one side of which the target sequence molecules are bound.

57632693, Jun 9, 1998

Feb 1, 1995

8/384476

Mark D. Cochran - Carlsbad CA
Meng-Fu Shih - San Diego CA
William P. MacConnell - Cardiff CA
Richard D. MacDonald - San Diego CA

Syntro Corporation - Lenexa KS

C12N 1586
C12N 1546

4353201

The present invention provides a hybrid, nonprimate herpesvirus comprising DNA which includes a sequence essential for viral replication of the hybrid, nonprimate herpesvirus, at least a portion of which is present in a sequence essential for replication of a naturally-occurring nonprimate herpesvirus and at least one foreign DNA sequence. Also provided is an attenuated, nonprimate herpesvirus comprising DNA which includes a sequence essential for viral replication of the attenuated, nonprimate herpesvirus, at least a portion of which is present in a sequence essential for replication of a naturally-occurring nonprimate herpesvirus, from which at least a portion of a repeat sequence has been deleted. Also provided are vaccines comprising the viruses of the invention and methods of immunizing animals against various disease.

58343059, Nov 10, 1998

Nov 4, 1994

8/334428

Mark D. Cochran - Carlsbad CA
William P. MacConnell - Cardiff CA
Richard D. MacDonald - San Diego CA
David E. Junker - San Diego CA

Syntro Corporation - Lenexa KS

C12N 1586

4353201

The present invention provides a hybrid, nonprimate herpesvirus comprising DNA which includes a sequence essential for viral replication of the hybrid, nonprimate herpesvirus, at least a portion of which is present in a sequence essential for replication of a naturally-occurring nonprimate herpesvirus and at least one foreign DNA sequence. Also provided is an attenuated, nonprimate herpesvirus comprising DNA which includes a sequence essential for viral replication of the attenuated, nonprimate herpes-virus, at least a portion of which is present in a sequence essential for replication of a naturally-occurring nonprimate herpesvirus, from which at least a portion of a repeat sequence has been deleted. Also provided are vaccines comprising the viruses of the invention and methods of immunizing animals against various disease.

62109618, Apr 3, 2001

Aug 18, 1997

8/912803

Mark D. Cochran - Carlsbad CA
Meng-Fu Shih - San Diego CA
William P. MacConnell - Cardiff CA
Richard D. Macdonald - San Diego CA

Syntro Corporation - San Diego CA

C12N 1586

4353201

The present invention provides a hybrid, nonprimate herpesvirus comprising DNA which includes a sequence essential for viral replication of the hybrid, nonprimate herpesvirus, at least a portion of which is present in a sequence essential for replication of a naturally-occurring nonprimate herpesvirus and at least one foreign DNA sequence. Also provided is an attenuated, nonprimate herpesvirus comprising DNA which includes a sequence essential for viral replication of the attenuated, nonprimate herpesvirus, at least a portion of which is present in a sequence essential for replication of a naturally-occurring nonprimate herpesvirus, from which at least a portion of a repeat sequence has been deleted. Also provided are vaccines comprising the viruses of the invention and methods of immunizing animals against various disease.

50681923, Nov 26, 1991

Jan 27, 1986

6/823102

Mark D. Cochran - La Jolla CA
Meng-Fu Shih - San Diego CA
William P. MacConnell - Cardiff CA

Prutech Research and Development Partnership - San Jose CA

C12N 1586

4352351

Attenuated pseudorabies viruses are provided which comprise DNA including a sequence essential for replication of the attenuated virus, at least a portion of which is present in a sequence essential for replication of a naturally-occurring pseudorabies virus, and at least one foreign DNA sequence adapted for expression in a host and encoding an amino acid sequence which is antigenic in the host. These viruses are useful as vaccines for immunizing animals against pseudorabies virus disease and other pathogens. The invention also provides methods of preparing attenuated pseudorabies viruses containing at least one foreign DNA sequence and methods of immunizing animals with vaccines comprised of these viruses.

61210439, Sep 19, 2000

Aug 15, 1997

8/915520

Mark D. Cochran - Carlsbad CA
Meng-Fu Shih - San Diego CA
William P. MacConnell - Cardiff CA
Richard D. Macdonald - San Diego CA

Syntro Corporation - San Diego CA

C12N 15869

4353201

The present invention provides a hybrid, nonprimate herpesvirus comprising DNA which includes a sequence essential for viral replication of the hybrid, nonprimate herpesvirus, at least a portion of which is present in a sequence essential for replication of a naturally-occurring nonprimate herpesvirus and at least one foreign DNA sequence. Also provided is an attenuated, nonprimate herpesvirus comprising DNA which includes a sequence essential for viral replication of the attenuated, nonprimate herpesvirus, at least a portion of which is present in a sequence essential for replication of a naturally-occurring nonprimate herpesvirus, from which at least a portion of a repeat sequence has been deleted. Also provided are vaccines comprising the viruses of the invention and methods of immunizing animals against various disease.

52175936, Jun 8, 1993

Jul 10, 1992

7/911515

William P. MacConnell - Cardiff CA

G01N 2726
G01N 27447

204299R

A process for the purification of DNA and the like comprises a housing having walls forming a reservoir having a chamber for containing a buffer solution, means for circulating a buffer through the reservoir, a disposable cassette within said chamber having first means including a gel for defining a first path extending between an inlet end and an outlet end, a well for introducing a bacterial sample into the path at said inlet end thereof, and a second path intersecting the first path via an elution chamber, having a collection chamber including an elution window at said outlet end, and an electrical circuit for selectively applying an electrical potential along each of the paths for selectively moving a plasmid first along the first path from the bacterial well to the elution chamber, then along the second path to the collection window at the end thereof.