Inventors:
Carl T. Wittwer - Salt Lake City UT, US
Luming Zhou - Salt Lake City UT, US
Virginie E. Dujols - Sandy UT, US
Joseph A. Holden - Salt Lake City UT, US
Assignee:
University of Utah Research Foundation - Salt Lake City UT
Idaho Technology, Inc. - Salt Lake City UT
International Classification:
C12Q 1/68
Abstract:
Methods are provided for nucleic acid analysis wherein a target nucleic acid is mixed with a dsDNA binding dye to form a mixture. Optionally, an unlabeled probe is included in the mixture. A melting curve is generated for the target nucleic acid by measuring fluorescence from the dsDNA binding dye as the mixture is heated. Dyes for use in nucleic acid analysis and methods for making dyes are also provided.