Thomas R Berkelman

7863048, Jan 4, 2011

Oct 19, 2009

12/581632

Thomas R. Berkelman - Oakland CA, US

Bio-Rad Laboratories, Inc. - Hercules CA

G01N 33/00
C07D 405/02

436 86, 546 66, 546 89, 546 99

Protein dyes whose molecular structure is that of a coumarin moiety coupled to a quaternary ammonium heterocycle through a vinyl or polyvinyl linkage demonstrate the ability to associate with proteins in a non-covalent, non-specific manner at low pH, where the associated form displays a significantly higher fluorescence emission than the unassociated form. This makes the dyes useful as selective labels for proteins at the low pH and eliminates the need for the removal of extraneous components from the medium in which the proteins reside prior to detection.

8282800, Oct 9, 2012

Sep 1, 2009

12/552104

Craig Rowell - Albany CA, US
Cory Panattoni - Winters CA, US
Thomas R. Berkelman - Oakland CA, US
Sean Cater - Oakland CA, US
Shane Petersen - Fairfield CA, US
Lee Olech - Pinole CA, US
Xuemei Yang - Walnut Creek CA, US

Bio-Rad Laboratories, Inc. - Hercules CA

G01N 27/447

204468, 204456, 204606

Polyacrylamide gels that offer high resolution in protein separations and are more stable relative to hydrolysis than conventional polyacrylamide gels that rely on Tris or Tris-Bis as buffering agents are made by incorporating triethanolamine in place of most or all of the Tris or Tris-Bis.

8536162, Sep 17, 2013

Mar 9, 2011

13/044220

Thomas R. Berkelman - Oakland CA, US
Lisandra Martin - Victoria, AU
Rhiannon Jones - Richmond, AU
John Walker, II - San Leandro CA, US

Bio-Rad Laboratories, Inc. - Hercules CA

A61K 31/33
C07D 255/02

514183, 540474

Conjugates of 1,3-bis(1,4,7-triazacyclonon-1-yl)-2-hydroxypropanes with a variety of conjugating members are used in the formation of dinuclear metal complexes which bind to phosphate esters. By virtue of their conjugated forms, the complexes are incorporated into chromatographic media, affinity binding reagents, and dyes, which make the complexes useful in a wide range of assays, separations, and purifications. In addition, dinuclear metal complexes of 1,3-bis(1,4,7-triazacyclonon-1-yl)-2-hydroxypropanes that are not so conjugated are used in the detection of phosphate esters of biological species by either MALDI-TOF mass spectrometry or by dye displacement.

20060166368, Jul 27, 2006

Jan 26, 2005

11/044798

Thomas Berkelman - Oakland CA, US

BIO-RAD LABORATORIES, INC. - Hercules CA

G01N 33/50

436086000

Protein dyes whose molecular structure is that of a coumarin moiety coupled to a quaternary ammonium heterocycle through a vinyl or polyvinyl linkage demonstrate the ability to associate with proteins in a non-covalent, non-specific manner at low pH, where the associated form displays a significantly higher fluorescence emission than the unassociated form. This makes the dyes useful as selective labels for proteins at the low pH and eliminates the need for the removal of extraneous components from the medium in which the proteins reside prior to detection.

20080091015, Apr 17, 2008

Aug 21, 2007

11/842779

Thomas Berkelman - Oakland CA, US
Sergiy Yarmoluk - Kyiv, UA
Vladyslava Kovalska - Kyiv, UA
Mykhaylo Losytskyy - Kyiv, UA
Kateryna Volkova - Kyiv, UA

Bio-Rad Laboratories, Inc. - Hercules CA

C07D 239/54
C07D 209/12

544301000, 548455000

Squaraine dyes are incorporated into a separation medium in which protein or polypeptide mixtures are separated, which medium also contains a detergent that forms a complex with the polypeptides or proteins. The dyes, upon excitation in the separation medium, exhibit a heretofore unrecognized selectivity in their fluorescent emissions by emitting a signal only when the dye molecules are associated with complexes of protein (or polypeptide) and detergent molecules, despite the additional presence of dyes in the bulk of the separation medium. The dyes are thus able to indicate the presence and locations of proteins or polypeptides in the separation medium without the need for removing unassociated dyes from the medium.

20100144046, Jun 10, 2010

Nov 30, 2009

12/627393

Thomas R. Berkelman - Oakland CA, US

Bio-Rad Laboratories, Inc. - Hercules CA

C07F 15/00
G01N 33/68

436 86, 546 4

A complex containing a transition metal ion and a plurality of donor ligands each of which is fully coordinated to the transition metal ion and is either a nitrogen donor ligand or a cyclometalated donor ligand, such that at least one of the donor ligands is a cyclometalated donor ligand bears one or more reactive groups connected to at least one of the donor ligands through a linker that includes a chain of four or more atoms. The linker offers advantages that make the complex particularly effective in labeling biomolecules and in multiplex analyses.

20120285830, Nov 15, 2012

Jul 23, 2012

13/555944

Craig Rowell - Albany CA, US
Cory Panattoni - Winters CA, US
Thomas R. Berkelman - Oakland CA, US
Sean Cater - Oakland CA, US
Shane Petersen - Fairfield CA, US
Lee Olech - Pinole CA, US
Xuemei Yang - Walnut Creek CA, US

Bio-Rad Laboratories - Hercules CA

C07K 1/26

204468

Polyacrylamide gels that offer high resolution in protein separations and are more stable relative to hydrolysis than conventional polyacrylamide gels that rely on Tris or Tris-Bis as buffering agents are made by incorporating triethanolamine in place of most or all of the Tris or Tris-Bis.

20140008226, Jan 9, 2014

Jul 3, 2013

13/934936

Cory Panattoni - Winters CA, US
Thomas R. Berkelman - Oakland CA, US
Sean Cater - Oakland CA, US
Shane Petersen - Fairfield CA, US
Lee Olech - Pinole CA, US
Xuemei Yang - Alamo CA, US

Bio-Rad Laboratories - Hercules CA

B01D 57/02

204469

Polyacrylamide gels that offer high resolution in protein separations and are more stable relative to hydrolysis than conventional polyacrylamide gels that rely on Tris or Tris-Bis as buffering agents are made by incorporating triethanolamine in place of most or all of the Tris or Tris-Bis.