Resumes
Resumes
Susanne Pippig Phones & Addresses
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San Francisco, CA
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Palo Alto, CA
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1495 7Th Ave APT 7, San Francisco, CA 94122 (510) 531-8173
Work
Position:
Administrative Support Occupations, Including Clerical Occupations
Education
Degree:
Associate degree or higher
Emails
Publications
Us Patents
Selectable Marker Genes
View pageUS Patent:
20020150876, Oct 17, 2002
Filed:
Mar 26, 2001
Appl. No.:
09/817487
Inventors:
Susanne Pippig - San Francisco CA, US
Gabor Veres - Palo Alto CA, US
Gabor Veres - Palo Alto CA, US
International Classification:
C12Q001/00
C12Q001/68
C12N015/867
G01N033/567
C12Q001/68
C12N015/867
G01N033/567
US Classification:
435/004000, 435/006000, 435/456000, 435/007200
Abstract:
The present invention concerns a method of using a muscle specific tyrosine kinase receptor molecule (MuSK-R) or a mutated MuSK-R (mMuSK-R) thereof as a selectable marker in mammalian cells, particularly human cells. Preferred markers are mMuSK-Rs incapable of signal transduction and preferably molecules wherein the intracellular domain has been modified by deletion of the signaling region. The invention also relates to a method of identifying genetically modified mammalian cells including introducing a mMuSK-R into a target cell as a selectable marker. Further a method for the immunoselection of transduced mammalian cells is disclosed comprising identifying the transduced cells by incubation of the cells with an antibody which recognizes and binds specifically to a MuSK-R or mMuSK-R thereof.
Selectable Cell Surface Marker Genes
View pageUS Patent:
6790614, Sep 14, 2004
Filed:
Nov 17, 2000
Appl. No.:
09/715249
Inventors:
Susanne Dagmar Pippig - San Francisco CA
Gabor Veres - Palo Alto CA
Gabor Veres - Palo Alto CA
Assignee:
Novartis AG - Basel
International Classification:
C12Q 168
US Classification:
435 6, 435 721, 435 723, 435 792, 435 691, 435183, 435325, 435332, 435455, 435456, 5303879, 53038826, 536 231
Abstract:
The present invention concerns a method of identifying genetically modified mammalian cells using a mutated protein-tyrosine kinase receptor (PTKR) as a selectable marker in mammalian cells. Particularly preferred mutated PTKR selective markers are mutated epidermal growth factor receptor (EGFR) family members, and muscle specific tyrosine kinase receptor (MuSK-R) family members. Further a method for the immunoselection of transduced mammalian cells is disclosed comprising retrovirally transducing mammalian cells with a nucleic acid sequence encoding a mutated EGFR, incubating the transduced cells with a marked antibody which recognizes and binds specifically to the mutated PTKR, and identifying the marked transduced cells.