US Patent:
20100099143, Apr 22, 2010
Inventors:
Ranjini Chatterjee - Belmont CA, US
Kenneth W. Mitchell - San Jose CA, US
Susan Y. Louie - San Francisco CA, US
Richard J. Fox - Kirkwood MO, US
Michelle Chen - Moorpark CA, US
International Classification:
C12P 21/02
C12N 9/10
C12N 15/11
C12N 15/00
C12N 1/21
US Classification:
435 691, 435193, 536 232, 4353201, 43525233
Abstract:
The present invention is directed to polypeptides that have enhanced alanine 2,3-aminomutase (AAM) activity and/or thermostability relative to the wild-type enzymes that have incidental AAM activity as a result of cross reactivity with alanine. In addition, the present invention is directed to a polynucleotides that encodes for the AAM polypeptides of the present invention, to nucleic acid sequences comprising the polynucleotides, to expression vectors comprising the polynucleotides operatively linked to a promoter, to host cells transformed to express the AAM polypeptides, and to a method for producing the AAM polypeptides of the present invention.