Sulekha Rao Coticone

6783940, Aug 31, 2004

Oct 31, 2001

09/998887

Ian J. McLaughlin - Moss Beach CA
Sulekha Rao Coticone - Half Moon Bay CA
Will Bloch - White Salmon WA

Applera Corporation - Foster City CA

C12Q 168

435 6, 435 71, 435 911, 435 912, 536 221, 536 231, 536 243, 536 2431, 536 2432, 536 2433

The invention provides methods for reducing non-specific amplification DNA in a polymerase chain reaction comprising providing a sample comprising a target DNA sequence of interest; contacting the sample with at least one enzyme having nucleic acid polymerase activity; and incubating the sample with said enzyme for a time and under conditions sufficient to amplify the target DNA sequence, forming amplified target sequence; wherein the incubation is performed in the presence of an amount of sorbitol, or sorbitol and DMSO effective to reduce the non-specific amplification relative to the amount of non-specific amplification observed in the absence of sorbitol, or sorbitol and DMSO. The methods are suitable for amplification of ribosomal DNA, particularly from clinical samples. Compositions and kits containing sorbitol, or sorbitol and DMSO for reducing non-specific amplification are also provided.

6841349, Jan 11, 2005

May 7, 2001

09/850514

Sulekha Rao Coticone - Half Moon Bay CA, US
Will Bloch - White Salmon WA, US

Applera Corporation Applied Biosystems Group - Foster City CA

C12Q 168
C07H 2102
C07H 2104

435 6, 536231, 536 243

The invention provides a method for reducing stutter in the amplification of a microsatellite comprising the steps of providing a sample comprising a microsatellite having a G+C content of 50% or less; contacting the sample with at least one enzyme having nucleic acid polymerase activity; and incubating the sample with the enzyme for a sufficient amount of time and under conditions sufficient to amplify the microsatellite; wherein the incubation is performed in the presence of an amount of betaine, sorbitol or mixtures thereof, effective to reduce stutter relative to the amount of stutter observed in the absence of betaine and/or sorbitol. The invention also provides compositions containing betaine and/or sorbitol, kits for amplifying microsatellites having a G+C content of 50% or less, and methods of using all of the foregoing.

7211385, May 1, 2007

Aug 8, 2003

10/637466

Sulekha Rao Coticone - Half Moon Bay CA, US
Will Bloch - Portland OR, US

Applera Corporation - Foster City CA

C12Q 1/68
C12P 19/34
C07H 21/02
C07H 21/04

435 6, 435 912, 536 231, 536 243, 536 2433

The invention provides a method for reducing stutter in the amplification of a microsatellite comprising the steps of providing a sample comprising a microsatellite having a G+C content of 50% or less; contacting the sample with at least one enzyme having nucleic acid polymerase activity; and incubating the sample with the enzyme for a sufficient amount of time and under conditions sufficient to amplify the microsatellite; wherein the incubation is performed in the presence of an amount of betaine, sorbitol or mixtures thereof, effective to reduce stutter relative to the amount of stutter observed in the absence of betaine and/or sorbitol. The invention also provides compositions containing betaine and/or sorbitol, kits for amplifying microsatellites having a G+C content of 50% or less, and methods of using all of the foregoing.

20020187475, Dec 12, 2002

May 7, 2001

09/850590

Sulekha Coticone - Half Moon Bay CA, US
Will Bloch - White Salmon WA, US

C12Q001/68
C12P019/34

435/006000, 435/091200

The invention provides a method for reducing stutter in the amplification of a microsatellite comprising the steps of providing a sample comprising a microsatellite having a G+C content of greater than 50%; contacting the sample with at least one enzyme having nucleic acid polymerase activity; and incubating the sample with the enzyme for a sufficient amount of time and under conditions sufficient to amplify the microsatellite; wherein the incubation is performed in the presence of an amount of sorbitol effective to reduce stutter relative to the amount of stutter observed in the absence of sorbitol. The invention also provides compositions containing sorbitol, kits for amplifying microsatellites having a G+C content of greater than 50%, and methods of using all of the foregoing.

20080213859, Sep 4, 2008

Apr 30, 2007

11/742524

Sulekha Rao Coticone - Half Moon Bay CA, US
Will Bloch - White Salmon WA, US

APPLERA CORPORATION - Foster City CA

C12N 9/10

435193

The invention provides a method for reducing stutter in the amplification of a microsatellite comprising the steps of providing a sample comprising a microsatellite having a G+C content of 50% or less; contacting the sample with at least one enzyme having nucleic acid polymerase activity; and incubating the sample with the enzyme for a sufficient amount of time and under conditions sufficient to amplify the microsatellite; wherein the incubation is performed in the presence of an amount of betaine, sorbitol or mixtures thereof, effective to reduce stutter relative to the amount of stutter observed in the absence of betaine and/or sorbitol. The invention also provides compositions containing betaine and/or sorbitol, kits for amplifying microsatellites having a G+C content of 50% or less, and methods of using all of the foregoing.

20110143352, Jun 16, 2011

Apr 28, 2010

12/769577

Sulekha Rao COTICONE - Half Moon Bay CA, US
Will Bloch - White Salmon WA, US

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

C12Q 1/68
C12N 9/12

435 612, 435194

The invention provides a method for reducing stutter in the amplification of a microsatellite comprising the steps of providing a sample comprising a microsatellite having a G+C content of 50% or less; contacting the sample with at least one enzyme having nucleic acid polymerase activity; and incubating the sample with the enzyme for a sufficient amount of time and under conditions sufficient to amplify the microsatellite; wherein the incubation is performed in the presence of an amount of betaine, sorbitol or mixtures thereof, effective to reduce stutter relative to the amount of stutter observed in the absence of betaine and/or sorbitol. The invention also provides compositions containing betaine and/or sorbitol, kits for amplifying microsatellites having a G+C content of 50% or less, and methods of using all of the foregoing.

20130011839, Jan 10, 2013

Sep 12, 2012

13/611974

Sulekha Rao Coticone - Naples FL, US
Will Bloch - White Salmon WA, US

Life Technologies Corporation - Carlsbad CA

C12Q 1/68
C12N 9/12
C12P 19/34

435 611, 435 912, 435194, 435 612

The invention provides a method for reducing stutter in the amplification of a microsatellite comprising the steps of providing a sample comprising a microsatellite having a G+C content of 50% or less; contacting the sample with at least one enzyme having nucleic acid polymerase activity; and incubating the sample with the enzyme for a sufficient amount of time and under conditions sufficient to amplify the microsatellite; wherein the incubation is performed in the presence of an amount of betaine, sorbitol or mixtures thereof, effective to reduce stutter relative to the amount of stutter observed in the absence of betaine and/or sorbitol. The invention also provides compositions containing betaine and/or sorbitol, kits for amplifying microsatellites having a G+C content of 50% or less, and methods of using all of the foregoing.