US Patent:
20140342932, Nov 20, 2014
Inventors:
- Rahway NJ, US
Byung-Kwon Choi - Norwich VT, US
Song Lin - Hanover NH, US
Natarajan Sethuraman - Hanover NH, US
Hussam Shaheen - Lebanon NH, US
Terrance Stadheim - Lyme NH, US
Dongxing Zha - Etha NH, US
International Classification:
G01N 33/74
US Classification:
506 9, 435 72, 435 732, 435 731, 435 721
Abstract:
Systems for making, identifying, and selecting recombinant cells that express a ligand for the insulin receptor (IR) or insulin growth factor I (IGF-1) receptor are described. In general, libraries of recombinant cells are constructed that are capable of displaying a plurality of ligand molecules on the cell surface. Recombinant cells that display a ligand in a form accessible for binding to the IR and/or IGF-1 receptor can be detected and the recombinant cells displaying said ligands can be selected and isolated using cell sorting technologies. In particular aspects, the system is useful for constructing and screening libraries of recombinant cells that express and displaying insulin analogue precursors molecules to identify and select recombinant cells in the library that bind the IR and/or IGF-1 receptor with a desired affinity and/or avidity.