Sohini Ghoshroy

Clark University
Worcester, MA
Apr 2011 to Mar 2014
Postdoctoral Research Fellow
Clark University
Worcester, MA
2003 to 2011
Doctoral Candidate

Clark University
Feb 2011
Ph. D. in Molecular Biology and Evolution
Parasitology University of Connecticut
2000
MS in Zoology
Parasitology University of Calcutta
Kolkata, West Bengal
1996
MS in Zoology
University of Calcutta, Presidency College
Kolkata, West Bengal
1994
BS in Zoology

Proteomics: Analyzing data from protein sequencing using Scaffold and DAVID Bioinformatics resources among other NCBI/ExPASy tools. Molecular Biology: DNA/RNA isolation, primer design, PCR, RT-PCR, qPCR, Rapid Amplification of cDNA ends (RACE) PCR. Cloning, restriction digests, sequencing with Big Dye v 3.1 with automated capillary sequencer ABI 3130 at Clark University. RNA protein interaction In vitro transcription of biotinylated RNA, Immuno- precipitation with biotinylated RNA-protein conjugate and streptavidin coated magnetic beads. Electrophoretic Mobility Shift Assays (EMSA). Silver staining methods. Protein methods: Western blotting, SDS-PAGE, Enzyme assay, spectrophotometry. Bioinformatics and phylogenetics: Data mining, sequence alignment and phylogenetic tree construction using Bayesian and Maximum likelihood inferences. Microscopy: Light microscopy. Dissection and Histology: Various vertebrate and invertebrate dissections. Tissue fixations, staining, slide preparations for mounting and sectioning. Culture maintenance and aseptic techniquesMaintenance of various strains of algal cultures under aseptic conditions. Photosynthesis: PAM fluorometry, pigment extraction and spectral quantification.