Sabine Dr Mohr from 4514 Avenue C, Austin, TX 78751, age 63, Phone: (512) 420-0168

Use Of Template Switching For Dna Synthesis

View page

US Patent:

20140004569, Jan 2, 2014

Filed:

Feb 23, 2012

Appl. No.:

14/000513

Inventors:

Alan M. Lambowitz - Austin TX, US
Sabine Mohr - Austin TX, US
Travis B. White - Austin TX, US
Scott Kuersten - Fitchburg WI, US

Assignee:

Board of Regents, The University of Texas System - Austin TX

International Classification:

C12P 19/34

US Classification:

435 9121, 435 912

Abstract:

A method of preparing a DNA copy of a target polynucleotide using template switching is described. The method includes mixing a double stranded template/primer substrate made up of a DNA primer oligonucleotide associated with a complementary oligonucleotide template strand with a target polynucleotide in a reaction medium and adding a suitable amount of a non-retroviral reverse transcriptase to the reaction medium to extend the DNA primer oligonucleotide from its 3′ end to provide a DNA copy polynucleotide. The DNA copy polynucleotide includes a complementary target DNA polynucleotide that is synthesized using the target polynucleotide as a template. Methods of adding nucleotides to the double stranded template/primer substrate are also described. The method can be used to facilitate detection, PCR amplification, cloning, and determination of RNA and DNA sequences.

Stabilized Reverse Transcriptase Fusion Proteins

View page

US Patent:

20120009630, Jan 12, 2012

Filed:

Mar 4, 2010

Appl. No.:

13/254223

Inventors:

Alan M. Lambowitz - Austin TX, US
Sabine Mohr - Austin TX, US
Georg Mohr - Austin TX, US
Eman Ghanem - Austin TX, US

Assignee:

Board of Regents, The University of Texas System - Austin TX

International Classification:

C12P 19/34
C12N 15/63
C12N 9/12

US Classification:

435 9151, 435194, 4353201

Abstract:

Stabilized reverse transcriptase fusion proteins including a thermostable reverse transcriptase connected to a stabilizer protein are described. Attaching the stabilizer protein to the thermostable reverse transcriptase stabilizes the fusion protein and can aid in its purification, provide increased solubility, allow for longer storage, or allow the fusion protein to be used under more rigorous conditions such as higher temperature. The stabilized reverse transcriptase fusion protein can also include a linker between the stabilizer protein and the thermostable reverse transcriptase. The stabilized reverse transcriptase fusion proteins are suitable for use in nucleic acid amplification methods such as the reverse transcription polymerase chain reaction and other applications involving cDNA synthesis.

Stabilized Reverse Transcriptase Fusion Proteins

View page

US Patent:

20180346890, Dec 6, 2018

Filed:

Aug 1, 2018

Appl. No.:

16/051549

Inventors:

- Austin TX, US
Sabine Mohr - Austin TX, US
Georg Mohr - Austin TX, US
Eman Ghanem - Raleigh NC, US

International Classification:

C12N 9/12
C12P 19/34

Abstract:

Stabilized reverse transcriptase fusion proteins including a thermostable reverse transcriptase connected to a stabilizer protein are described. Attaching the stabilizer protein to the thermostable reverse transcriptase stabilizes the fusion protein and can aid in its purification, provide increased solubility, allow for longer storage, or allow the fusion protein to be used under more rigorous conditions such as higher temperature. The stabilized reverse transcriptase fusion protein can also include a linker between the stabilizer protein and the thermostable reverse transcriptase. The stabilized reverse transcriptase fusion proteins are suitable for use in nucleic acid amplification methods such as the reverse transcription polymerase chain reaction and other applications involving cDNA synthesis.

Stabilized Reverse Transcriptase Fusion Proteins

View page

US Patent:

20180340158, Nov 29, 2018

Filed:

Aug 1, 2018

Appl. No.:

16/051544

Inventors:

- Austin TX, US
Sabine Mohr - Austin TX, US
Georg Mohr - Austin TX, US
Eman Ghanem - Raleigh NC, US

International Classification:

C12N 9/12
C12P 19/34

Abstract:

Stabilized reverse transcriptase fusion proteins including a thermostable reverse transcriptase connected to a stabilizer protein are described. Attaching the stabilizer protein to the thermostable reverse transcriptase stabilizes the fusion protein and can aid in its purification, provide increased solubility, allow for longer storage, or allow the fusion protein to be used under more rigorous conditions such as higher temperature. The stabilized reverse transcriptase fusion protein can also include a linker between the stabilizer protein and the thermostable reverse transcriptase. The stabilized reverse transcriptase fusion proteins are suitable for use in nucleic acid amplification methods such as the reverse transcription polymerase chain reaction and other applications involving cDNA synthesis.

Stabilized Reverse Transcriptase Fustion Proteins

View page

US Patent:

20160289652, Oct 6, 2016

Filed:

May 25, 2016

Appl. No.:

15/164208

Inventors:

- Austin TX, US
Sabine Mohr - Austin TX, US
Georg Mohr - Austin TX, US
Eman Ghanem - Austin TX, US

International Classification:

C12N 9/12
C12P 19/34

Abstract:

Stabilized reverse transcriptase fusion proteins including a thermostable reverse transcriptase connected to a stabilizer protein are described. Attaching the stabilizer protein to the thermostable reverse transcriptase stabilizes the fusion protein and can aid in its purification, provide increased solubility, allow for longer storage, or allow the fusion protein to be used under more rigorous conditions such as higher temperature. The stabilized reverse transcriptase fusion protein can also include a linker between the stabilizer protein and the thermostable reverse temperature. The stabilized reverse transcriptase fusion proteins are suitable for use in nucleic acid amplification methods such as the reverse transcription polymerase chain reaction and other applications involving cDNA synthesis.