Ronald Lollar Phones & Addresses
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8096 Rolling Hills Dr, Athens, OH 45701
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1182 Wenonah Ave, Oak Park, IL 60304
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Carpentersville, IL
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Berry, AL
Publications
Us Patents
Methods For Direct Fluorescent Antibody Virus Detection In Liquids
View pageUS Patent:
20100261153, Oct 14, 2010
Filed:
Apr 16, 2009
Appl. No.:
12/425256
Inventors:
David R. Scholl - Athens OH, US
James L. Brown - Athens OH, US
Ronald Lollar - Athens OH, US
James L. Brown - Athens OH, US
Ronald Lollar - Athens OH, US
International Classification:
C12Q 1/70
US Classification:
435 5
Abstract:
The present invention describes a liquid direct fluorescence antibody assay that is rapid and sensitive to detect respiratory virus in infected cells. The assay includes centrifugation of the specimen, incubation of sample and reagents in solution, and detection of the absence or presence of respiratory virus. Sapogenin is used as a detergent to permeabilize the cells for entry of the monoclonal antibodies to react with intracellular antigens. The cells are stained with fluorescently labeled monoclonal antibodies against the viral antigens along with a background stain and a fluorescent nuclear stain. This counter staining decreases background and allows co-localization of antigen and nuclear structures for enhanced detection.
Methods For Direct Fluorescent Antibody Virus Detection In Liquids
View pageUS Patent:
20110269117, Nov 3, 2011
Filed:
Jun 22, 2011
Appl. No.:
13/166228
Inventors:
David R. Scholl - Athens OH, US
James L. Brown - Athens OH, US
Ronald Lollar - Athens OH, US
James L. Brown - Athens OH, US
Ronald Lollar - Athens OH, US
International Classification:
C12Q 1/70
US Classification:
435 5
Abstract:
The present invention describes a liquid direct fluorescence antibody assay that is rapid and sensitive to detect respiratory virus in infected cells. The assay includes centrifugation of the specimen, incubation of sample and reagents in solution, and detection of the absence or presence of respiratory virus. Sapogenin is used as a detergent to permeabilize the cells for entry of the monoclonal antibodies to react with intracellular antigens. The cells are stained with fluorescently labeled monoclonal antibodies against the viral antigens along with a background stain and a fluorescent nuclear stain. This counter staining decreases background and allows co-localization of antigen and nuclear structures for enhanced detection.
Portable Fluorescence Reader Device
View pageUS Patent:
20120003627, Jan 5, 2012
Filed:
Jun 23, 2011
Appl. No.:
13/167223
Inventors:
David R. Scholl - Athens OH, US
James L. Brown - Athens OH, US
Ronald H. Lollar - Athens OH, US
Brooke B. McCutchan - Athens OH, US
James L. Brown - Athens OH, US
Ronald H. Lollar - Athens OH, US
Brooke B. McCutchan - Athens OH, US
International Classification:
C12Q 1/70
C12M 1/34
C12M 1/34
US Classification:
435 5, 4352872
Abstract:
The present invention describes a device for performing a liquid direct fluorescence antibody assay that is rapid and sensitive to detect respiratory virus in infected cells. The device also includes a compatible slide comprising sample wells. The device detects emitted fluorescence signal through a camera and optics assembly that is controlled by a user interface assembly.
Methods For Liquid Direct Fluorescent Antibody Intracellular Virus Detection
View pageUS Patent:
20150192584, Jul 9, 2015
Filed:
Jan 21, 2015
Appl. No.:
14/601647
Inventors:
- Athens OH, US
James L. Brown - Athens OH, US
Ronald Lollar - Athens OH, US
James L. Brown - Athens OH, US
Ronald Lollar - Athens OH, US
International Classification:
G01N 33/569
Abstract:
The present invention describes a liquid direct fluorescence antibody assay that is rapid and sensitive to detect respiratory virus in infected cells. The assay includes centrifugation of the specimen, incubation of sample and reagents in solution, and detection of the absence or presence of respiratory virus. Sapogenin is used as a detergent to permeabilize the cells for entry of the monoclonal antibodies to react with intracellular antigens. The cells are stained with fluorescently labeled monoclonal antibodies against the viral antigens along with a background stain and a fluorescent nuclear stain. This counter staining decreases background and allows co-localization of antigen and nuclear structures for enhanced detection.