Inventors:
Joseph G. Zeikus - Madison WI
Raphael J. Lamed - Schenectady NY
Assignee:
Wisconsin Alumni Research Foundation - Madison WI
International Classification:
C12N 902
C12N 904
C12Q 132
Abstract:
A partially purified NADP specific thermostable alcohol, aldehyde/ketone oxidoreductase is prepared which can react with a wide range of alcohols, ketones and aldehydes. The enzyme has a unique preference for secondary over primary alcohols. The thermostability, broad range of operating temperatures and lack of sensitivity to metal ions and complexing agents, in addition to the absolute specifity for the coenzyme, increase the utility of the enzyme in asymmetric organic synthesis and NADPH regeneration.