Rakhee Sachdeva

New York, NY

Research

Columbia University Medical Center - Greater New York City Area since Apr 2009
Postdoctoral Research Scientist
Postgraduate Institute of Medical Education and Research - Chandigarh Area, India Nov 2008 - Mar 2009
Research Associate

Postgraduate Institute of Medical Education and Research 2004 - 2008
PhD, Molecular Immunology and Parasitology Central Research Institute Kasauli 2001 - 2003
MSc, Microbiology

Molecular Biology
Patent Law
Scientific Writing
Prior Art Search
Scientific Communications
Intellectual Property
Immunology
Cell Biology
Cell Culture
Assay Development
Molecular Cloning
Western Blotting
Transfection
Flow Cytometry
Elisa
In Vivo
Confocal Microscopy
Pcr
Tissue Culture
Immunofluorescence
Protein Expression
Protein Purification
Cell
Qpcr
Immunoprecipitation
Fluorescence Microscopy
Microbiology
Emsa
Rna Isolation
Immunohistochemistry
Animal Models
Dna
Chip
Virus Culture
Facs
Microscopy
Sds Page
Bacterial Culture
Genetics
Molecular Genetics
Virology
Mammalian Cell Culture
Sequence Analysis
In Vitro
Real Time Pcr
Proteomics
Bacterial Transformation
Genotyping
Rnai
Biotechnology
Microsoft Excel
Business Analytics
Financial Accounting
Business Economics

English
Hindi
Punjabi

Intellectual Property Fundamentals
Business Writing Fundamentals
Patent Fundamentals Bootcamp: An Introduction To Patent Drafting, Prosecution, and Litigation
Understanding Patent Law 2017

St. Luke's-Roosevelt Institute for Health Sciences

2013 to 2000
Assistant Research Scientist
Columbia University
New York, NY
May 2009 to Jul 2013
Post-Doctoral Research Scientist

Department of Parasitology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Chandigarh, Chandigarh
2004 to 2008
Ph.D. in Molecular Microbiology

Cell culture techniques: Single Cell Cloning, Maintenance and preservation of adherent and suspension cell cultures, Experience working with Primary cells, maintenance of amastigotes and promastigotes of Leishmania, malarial parasite in blood culture, Infectivity assays, cytotoxicity assays, growing viruses like HIV, measles, mumps, rubella, performing Transient and stable transfections. Virus production: Making and maintaining stocks of viruses, such as HIV, EcoHIV, and EcoNDK. Molecular Biology/Immunological methods: Genomic and Plasmid DNA isolation, purification and their estimation; PCR amplification, RealTime-PCR, Reverse Transcriptase-PCR, PCR technique by Step-Oing method, Molecular Cloning, Restriction digestion, Site-Directed Mutagenesis, development of siRNA, oligo-end labeling, southern hybridization, DNA Sequencing analysis, Gel Shift assays (EMSA), Western blotting, Northern Blotting, CHIP assays, Immunoprecipitation, Co- Immunoprecipitation, , Immunofluorescence, Rapid Suppression Assay, Radiolabelling, Scintillation Counting, Radioactive Phospho-Imaging., MAGI assays, All basic hematological techniques, ELISA, Flow Cytometry and FACS analysis, Splenocyte proliferation, Cytokines assays for Th1 and Th2 responses, Protein purification: Small scale purification of fused proteins such as GST and His tag proteins Bioinformatics: Use of databases such as ChromasPro, miRbase, NCBI-blast, ClustalW, Treeview, DNA/protein sequence analysis, GraFit, Sigmaplot and other web based bioinformatics tools for epidemiological analysis Microbiology: Classical Microbiological techniques like Streaking, Spreading, Dilution Plating, etc and staining techniques such as Negative Staining, Gram Staining, Zeil Neilson Staining, Fermentation technologies, Handling of pathogenic bacteria, viruses and parasites, isolation; identification and preservation of microorganisms, antibiotic susceptibility testing, bacterial adherence and invasion assay Microscopy: Confocal microscopy, Fluorescence Microscopy.