Inventors:
Alfred L. Goldberg - Brookline MA
Olga Kandror - Newton MA
Assignee:
President and Fellows of Harvard College - Cambridge MA
International Classification:
C12N 990
Abstract:
Methods are disclosed which employ cold-shock proteins, such as Trigger Factor, to confer cold-tolerance to cells and to enhance viability of cells at temperatures at which the cells would not normally remain viable. Proteins having peptidyl-proline isomerase activity are also disclosed as being useful to confer cold-tolerance to cells. Cells which overexpress cold-shock proteins are useful in cell-based expression systems which are intended to express proteins at low temperatures.