Melaku M Woldemariam

20230035221, Feb 2, 2023

Mar 16, 2022

17/696180

- Philadelphia PA, US
Melaku Muluneh Woldemariam - Philadelphia PA, US

B01L 3/00
B03C 1/035
B03C 1/28
G01N 33/569
G01N 33/574

A magnetic separation device has a membrane having a plurality of pores, a magnetically soft material layer disposed on the membrane, and a passivation layer disposed on the magnetically soft material layer. The magnetic separation device may be part of a microfluidic device having a lateral flow channel and a vertical flow magnetic separation filter. The magnetic separation device may be used to separate magnetically tagged particles, such as cells.

20230020683, Jan 19, 2023

Jan 26, 2021

17/757745

- Tarrytown NY, US
Melaku Woldemariam - San Leandro CA, US
Ben Blizard - Oakland CA, US

Siemens Healthcare Diagnostics Inc. - Tarrytown NY

C12N 15/10
B03C 1/01
B03C 1/033
B03C 1/28
B01F 33/451

An apparatus and method for extracting nucleic acids such as DNA molecules from biological samples uses solid-state magnetic manipulation. A fluid sample and magnetic beads are placed in a vessel. The vessel is placed in a housing with an array of electromagnets mounted therein. The electromagnets are energized sequentially or in groups to move the magnetic beads through the fluid sample in a variety of patterns. The apparatus disclosed herein may be used as a measurement device to measure bead number density and modify magnetic patterns in order to deliver consistent dosages in bead number.

20200200679, Jun 25, 2020

Oct 1, 2019

16/589516

- Philadelphia PA, US
Melaku Muluneh Woldemariam - Philadelphia PA, US

G01N 21/64
B01L 3/00

Microfluidic devices for analyzing droplets are disclosed. A described microfluidic device includes a substrate and a microfluidic channel formed on the substrate. The microfluidic channel includes passages where each passage has a mask pattern configured to modulate a signal of a droplet passing through that passage, such that droplets passing through the passages produce signals. The microfluidic device also includes a detector configured to detect the signals. Methods of analyzing droplets with a microfluidic device having a microfluidic channel formed on a substrate are disclosed. A described method includes passing droplets through the passages, modulating signals from the droplets using mask patterns formed on the passages; and detecting the signals.

20190336973, Nov 7, 2019

May 14, 2019

16/411284

- Philadelphia PA, US
Melaku Muluneh Woldemariam - Philadelphia PA, US

B01L 3/00
B03C 1/28
G01N 33/569
G01N 33/574
B03C 1/035

A magnetic separation device has a membrane having a plurality of pores, a magnetically soft material layer disposed on the membrane, and a passivation layer disposed on the magnetically soft material layer. The magnetic separation device may be part of a microfluidic device having a lateral flow channel and a vertical flow magnetic separation filter. The magnetic separation device may be used to separate magnetically tagged particles, such as cells.

20190275523, Sep 12, 2019

Mar 20, 2019

16/359368

- Philadelphia PA, US
Melaku Muluneh Woldemariam - Philadelphia PA, US

B01L 3/00
B01F 15/00
B01F 13/10
B01F 13/00
B01F 3/08

A microfluidic device contains a first layer having a plurality of channels, a second layer having a plurality of droplet makers, and a third layer having a plurality of through-holes connecting the plurality of channels to the plurality of droplet makers. The channels have a height of at least 4 times greater than the height of the droplet makers. The microfluidic device has at least 500 droplet makers in an area less than 10 cm. The channels are formed by direct laser-micromachining and the droplet makers are formed by soft lithography molding.

20160271609, Sep 22, 2016

Oct 1, 2014

14/900759

- Philadelphia PA, US
MELAKU MULUNEH WOLDEMARIAM - PHILADELPHIA PA, US

B01L 3/00
B01F 3/08
B01F 15/00
B01F 13/00

A microfluidic device contains a first layer having a plurality of channels, a second layer having a plurality of droplet makers, and a third layer having a plurality of through-holes connecting the plurality of channels to the plurality of droplet makers. The channels have a height at least 4 times greater than the height of the droplet makers. The microfluidic device has at least 500 droplet makers in an area less than 10 cm. The channels are formed by direct laser-micromachining and the droplet makers are formed by soft lithography molding.

20160158756, Jun 9, 2016

Jul 23, 2014

14/907720

- Philadelphia PA, US
MELAKU MULUNEH WOLDEMARIAM - PHILADELPHIA PA, US

THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA - PHILADELPHIA PA

B01L 3/00
G01N 33/574
G01N 33/569

A magnetic separation device has a membrane having a plurality of pores, a magnetically soft material layer disposed on the membrane, and a passivation layer disposed on the magnetically soft material layer. The magnetic separation device may be part of a microfluidic device having a lateral flow channel and a vertical flow magnetic separation filter. The magnetic separation device may be used to separate magnetically tagged particles, such as cells.