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Maurizio Pellegrino

from Berkeley, CA
Age ~44
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Also known as:
Brian Herlihy, Mauricio Pelligrino
Connected to:
Alejandro Pellegrini, 38Amanda M Pellegrine, 46Amie R Pellegrini, 41Ana Pellegrini, 62Annmarie I Pellegrino, 69Antonietta Pellegrino, 58August A Pellegrini, 96Barbara Pellegrino, 87Barbara W Pellegrin, 69Bradley D Pellegrino, 51

Maurizio Pellegrino Phones & Addresses

  • Berkeley, CA
  • Knightdale, NC
  • Oakland, CA
  • San Francisco, CA
  • New York, NY
  • 320 E 53Rd St, New York, NY 10022

Skills

Laboratory • Qpcr • Cell Biology • Emulsions • Neuroscience • Genomics • Biotechnology • Cell Culture • Molecular Biology • Rna Isolation • Microfluidics • Bioinformatics • Immunohistochemistry • Sequence Analysis • Rt Pcr • Protein Purification • Genetics • Biochemistry • Science

Resumes

Resumes

Maurizio Pellegrino Photo 1

Maurizio Pellegrino

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Skills:
Laboratory
Qpcr
Cell Biology
Emulsions
Neuroscience
Genomics
Biotechnology
Cell Culture
Molecular Biology
Rna Isolation
Microfluidics
Bioinformatics
Immunohistochemistry
Sequence Analysis
Rt Pcr
Protein Purification
Genetics
Biochemistry
Science

Publications

Us Patents

Method, Apparatus And System To Detect Indels And Tandem Duplications Using Single Cell Dna Sequencing

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US Patent:
20210027859, Jan 28, 2021
Filed:
Jul 22, 2020
Appl. No.:
16/936382
Inventors:
- South San Francisco CA, US
Maurizio Pellegrino - San Francisco CA, US
International Classification:
G16B 30/00
G16B 20/20
G16B 20/50
G16B 35/00
Abstract:
The disclosure generally relates to method, apparatus and system to detect indels and tandem duplications using single cell DNA sequencing. An exemplary method to detect one or more indel variants in a single cell DNA sequence may include the steps of: (1) obtaining a plurality of sequenced data sets from a cell sample having one or more indel variants, each of the plurality of sequenced data sets further includes a forward-direction sequencing read (R) and a reverse-direction sequencing read (R); (2) processing the plurality of sequenced data sets to identify a region of interest (ROI) in the forward-direction sequencing read (R) and in the reverse-direction sequencing read (R) for each of the plurality of sequenced data; (3) mapping each ROI to a known genome to identify target loci in each of Rand Rthat do not map to the genome; (4) selecting a subset of the mapped ROIs with acceptable reads to identify a group of cells of interest; (5) from the selected subset, identifying one or more soft-clipped reads each ROI to identify a group of indel variants; and (6) determining at least one of location or frequency of occurrence for each indel variant of the identified group with respect to the corresponding ROI.

Method, Systems And Apparatus For High-Throughput Single-Cell Dna Sequencing With Droplet Microfluidics

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US Patent:
20190112655, Apr 18, 2019
Filed:
Oct 18, 2018
Appl. No.:
16/164595
Inventors:
- South San Francisco CA, US
Adam R. Sciambi - South San Francisco CA, US
Maurizio Pellegrino - South San Francisco CA, US
Assignee:
Mission Bio, Inc. - South San Francisco CA
International Classification:
C12Q 1/6874
Abstract:
The disclosed embodiments relate to method, apparatus and system for high throughput single-cell DNA sequencing with droplet microfluidic. In an exemplary embodiment, a microfluidic apparatus is used to provide a rapid and cost-effective targeted genomic sequencing of thousands of cells in parallel. The targeted sequencing can be directed for residual disease detection. In one embodiment, the disclosure provides a method to detect one or more mutations in tumor cells, the method comprising: encapsulating at least one cell and a lysis reagent in a carrier fluid to form a droplet, wherein the cell originates from a tumor and the cell comprises a genomic DNA; lysing the cell to release the genomic DNA and thereby form a droplet containing the genomic DNA; introducing a cell identifier and one or more primers specific to a plurality of regions of the genomic DNA; and thermocycling the droplet to amplify the genomic DNA and to incorporate cell identifiers into the genomic DNA to produce a plurality of amplified DNA with identified loci; wherein once the cell identifier is incorporated into the amplified DNA, the identified loci are sequenced and at least one DNA mutation is identified for the tumor cells.
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Maurizio Pellegrino from Berkeley, CA, age ~44 Get Report