US Patent:
20050233428, Oct 20, 2005
Inventors:
Ronald Vale - San Francisco CA, US
Kurt Thorn - San Francisco CA, US
Roger Cooke - San Francisco CA, US
Marija Matuska - San Francisco CA, US
Nariman Naber - San Bruno CA, US
International Classification:
C12N009/00
C12P021/06
Abstract:
The present invention features methods for purifying polypeptides of interest using a modified Fluorescein arsenical helix binder (FlAsH) compound immobilized on a solid support. An exemplary FlAsH target sequence motif is also presented. Examples of modification of the FlAsH compound which allow immobilization to a solid support are also provided. The present invention also provides DNA constructs for producing a dual affinity tagged polypeptide and methods for purification thereof.
