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Lee Scott Basehore

from Lakeside, CA
Age ~63

Lee Basehore Phones & Addresses

  • 11147 Oak Creek Dr, Lakeside, CA 92040 (619) 867-8100
  • La Jolla, CA
  • Escondido, CA
  • San Diego, CA
  • 11147 Oak Creek Dr, Lakeside, CA 92040

Work

Company: Stratagene 1986 to 2008 Position: Lab monkey

Skills

Biotechnology • Molecular Biology • Genomics • Lifesciences • Pcr • Microbiology • Assay Development • Life Sciences • R&D • Research • Drug Discovery • Dna Sequencing • Biochemistry • Protein Chemistry • Process Improvement • Cross Functional Team Leadership • Data Analysis • Biopharmaceuticals • Genetics • Polymerase Chain Reaction • Research and Development

Languages

English

Interests

Exercise • Sweepstakes • Nascar • Home Improvement • Scuba Diving • Reading • Sports • Fishing • Home Decoration • Watching Sports • Cooking • Skiing • Electronics • Outdoors • Baseball • Crafts • Music • Camping • Dogs • Family Values • Movies • Collecting • Christianity • Automobiles • Cats • Woodwork • Travel • Career • Watching Baseball • Investing • Traveling • Stamp Collecting

Industries

Biotechnology

Resumes

Resumes

Lee Basehore Photo 1

Research Scientist

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Location:
11147 Oak Creek Dr, Lakeside, CA 92040
Industry:
Biotechnology
Work:
Stratagene 1986 - 2008
Lab Monkey

Agilent Technologies 1986 - 2008
Research Scientist

Stratagene 1986 - 2008
Research Scientist
Skills:
Biotechnology
Molecular Biology
Genomics
Lifesciences
Pcr
Microbiology
Assay Development
Life Sciences
R&D
Research
Drug Discovery
Dna Sequencing
Biochemistry
Protein Chemistry
Process Improvement
Cross Functional Team Leadership
Data Analysis
Biopharmaceuticals
Genetics
Polymerase Chain Reaction
Research and Development
Interests:
Exercise
Sweepstakes
Nascar
Home Improvement
Scuba Diving
Reading
Sports
Fishing
Home Decoration
Watching Sports
Cooking
Skiing
Electronics
Outdoors
Baseball
Crafts
Music
Camping
Dogs
Family Values
Movies
Collecting
Christianity
Automobiles
Cats
Woodwork
Travel
Career
Watching Baseball
Investing
Traveling
Stamp Collecting
Languages:
English

Publications

Us Patents

Compositions And Methods For Protein Isolation

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US Patent:
7238478, Jul 3, 2007
Filed:
Nov 13, 2003
Appl. No.:
10/712137
Inventors:
Jeffrey C. Braman - Carlsbad CA, US
Carsten-Peter Carstens - San Diego CA, US
Natalia Novoradovskaya - San Diego CA, US
Rajesh Bagga - San Diego CA, US
Lee Scott Basehore - Lakeside CA, US
Assignee:
Stratagene California - La Jolla CA
International Classification:
C12Q 1/68
G01N 33/53
C12P 21/02
C12N 15/63
C07K 14/00
C07K 1/22
US Classification:
435 6, 435 71, 435 691, 4352523, 43525411, 4353201, 435325, 530350, 530413
Abstract:
The invention provides for polynucleotides and vectors comprising at least two tag sequences. The invention also provides for polynucleotides and vectors comprising a streptavidin binding peptide sequence and a calmodulin binding peptide sequence. The invention also provides for polynucleotides and vectors wherein a gene of interest is fused in frame to at least two tag sequences, for example, a streptavidin binding peptide sequence and a calmodulin binding peptide sequence. The invention also provides for the chimeric proteins encoded by these polynucleotides. The invention also provides for methods of using the polynucleotides of the invention for detecting and/isolating protein complexes or identifying a binding partner for a protein of interest.

Compositions And Methods For Protein Isolation

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US Patent:
20050158711, Jul 21, 2005
Filed:
Nov 12, 2004
Appl. No.:
10/987388
Inventors:
Jeffrey Braman - Carlsbad CA, US
Carsten-Peter Carstens - San Diego CA, US
Natalia Novoradovskaya - San Diego CA, US
Rajesh Bagga - San Diego CA, US
Lee Basehore - Lakeside CA, US
International Classification:
C12Q001/70
C12Q001/68
C12N007/00
C12N015/86
US Classification:
435005000, 435006000, 435456000, 435235100
Abstract:
The invention provides for polynucleotides and vectors comprising at least two tag sequences. In particular, preferred vectors are viral vectors. The invention also provides for polynucleotides and vectors comprising a streptavidin binding peptide sequence and a calmodulin binding peptide sequence. The invention also provides for polynucleotides and vectors wherein a gene of interest is fused in frame to at least two tag sequences, for example, a streptavidin binding peptide sequence and a calmodulin binding peptide sequence. The invention also provides for methods of using the polynucleotides and vectors of the invention for detecting and/isolating protein complexes or identifying a binding partner for a protein of interest.

Combined Lysis And Pcr Buffer

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US Patent:
20060286557, Dec 21, 2006
Filed:
Jun 15, 2005
Appl. No.:
11/152773
Inventors:
Lee Basehore - Lakeside CA, US
Natalia Novoradovskaya - San Diego CA, US
Jeffery Braman - Carlsbad CA, US
International Classification:
C12Q 1/68
C12P 19/34
US Classification:
435006000, 435091200, 435270000
Abstract:
The present invention provides compositions, methods, and kits for lysing cells, storing nucleic acids, amplifying nucleic, and analyzing nucleic acids. Among other things, the compositions, methods, and kits are suitable for one-step lysis and amplification of nucleic acid sequences of interest. In general, the compositions comprise TCEP and a non-ionic detergent, such as Triton X-100.

Normalization Of Samples For Amplification Reactions

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US Patent:
20060286558, Dec 21, 2006
Filed:
Jun 15, 2005
Appl. No.:
11/152775
Inventors:
Natalia Novoradovskaya - San Diego CA, US
Alexey Novoradovsky - San Diego CA, US
Joseph Sorge - Del Mar CA, US
Lee Basehore - Lakeside CA, US
Jeffery Braman - Carlsbad CA, US
International Classification:
C12Q 1/68
C07H 21/04
C12P 19/34
US Classification:
435006000, 435091200, 536024300
Abstract:
The present invention provides oligonucleotide primers, compositions, methods, and kits for determining the number of cells from which a cell lysate originated. The invention relies on detection and amplification of unique genomic sequences within the genome of an organism of interest to determine the amount of genomic nucleic acid in a sample. The invention can be used to normalize samples from particular cells, cell types, or organism, and provide an accurate basis for comparison of expression of genes across the samples.

Normalization Of Samples For Amplification Reactions

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US Patent:
20070003955, Jan 4, 2007
Filed:
Jun 15, 2006
Appl. No.:
11/453262
Inventors:
Natalia Novoradovskaya - San Diego CA, US
Alexey Novoradovsky - San Diego CA, US
Joseph Sorge - Del Mar CA, US
Lee Basehore - Lakeside CA, US
Jeffery Braman - Carlsbad CA, US
International Classification:
C12Q 1/68
C12P 19/34
US Classification:
435006000, 435091200
Abstract:
The present invention provides oligonucleotide primers, compositions, methods, and kits for determining the amount of gDNA or the number of cells from which a cell lysate originated. The invention relies on detection and amplification of unique genomic sequences within the genome of an organism of interest to determine the amount of genomic nucleic acid in a sample. The invention can be used to normalize samples from particular cells, cell types, or organism, and provide an accurate basis for comparison of expression of genes across the samples.

Lysis And Stabilization Buffer Suitable For Inclusion In Pcr Reactions

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US Patent:
20070015185, Jan 18, 2007
Filed:
Jun 15, 2006
Appl. No.:
11/453263
Inventors:
Lee Basehore - Lakeside CA, US
Natalia Novoradovskaya - San Diego CA, US
Jeffrey Braman - Carlsbad CA, US
International Classification:
C12Q 1/68
C12P 19/34
C12N 1/08
C07H 21/04
US Classification:
435006000, 435091200, 435270000, 536025400
Abstract:
The present invention provides compositions, methods, and kits for lysing cells, storing nucleic acids, amplifying nucleic acids, and analyzing nucleic acids. Among other things, the compositions, methods, and kits are suitable for one-step lysis and amplification of nucleic acid sequences of interest. In general, the compositions comprise TCEP and a non-ionic surfactant, such as Triton X-100.

System For Isolating Biomolecules From A Sample

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US Patent:
20080026451, Jan 31, 2008
Filed:
Jun 15, 2007
Appl. No.:
11/764117
Inventors:
Jeffrey Braman - Carlsbad CA, US
Lee Basehore - Lakeside CA, US
Natalia Novoradovskaya - San Diego CA, US
International Classification:
C12N 1/08
C12M 1/00
US Classification:
435270000, 435286500
Abstract:
The present invention provides an automated system for purification of a substance of interest. The system generally comprises an instrument for moving fluids through the system, a reagent pack for storing fluids, and a purification cartridge. The cartridge comprises two filtration units for binding substances based on different physical properties. The cartridge also comprises rotary valves for control of movement of fluids on the cartridge. In preferred embodiments, the system is useful for purifying RNA from blood samples.

Methods For The Separation Of Biological Molecules Using Dioxolane

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US Patent:
20080146789, Jun 19, 2008
Filed:
Mar 20, 2007
Appl. No.:
11/688662
Inventors:
Jeffrey C. BRAMAN - Carlsbad CA, US
Lee S. Basehore - Lakeside CA, US
Natalia Novoradovskaya - San Diego CA, US
International Classification:
C07H 21/00
C07K 1/00
C12N 9/00
US Classification:
536 253, 435183, 530412
Abstract:
The present invention provides a method for the isolation of biological molecules by the adsorption of the molecules onto a mineral substrate in the presence of an appropriate mixture of salts and dioxolane. Preferably, the biological molecules are nucleic acids. Compositions and kits for performing the process according to the invention are also provided.
Lee Scott Basehore from Lakeside, CA, age ~63 Get Report