Kent E Goklen

7241866, Jul 10, 2007

Oct 24, 2003

10/526301

Kent E. Goklen - Fanwood NJ, US
Joseph Nti-Gyabaah - Somerset NJ, US
Firoz D. Antia - Montclair NJ, US
Mary Ellen Dahlgren - Metuchen NJ, US

Merck & Co., Inc. - Rahway NJ

C07K 1/00

530350

This invention relates to the use of amine, amino acid and amino acid ester mobile modifiers in normal phase chromatography to improve the resolution and or productivity of peptide and lipopeptide purification. This chromatographic method can be sued for either analytical or preparative scale purification.

20020028916, Mar 7, 2002

Aug 1, 2001

09/920497

Martin Chandler - East Brunswick NJ, US
Kent Goklen - Fanwood NJ, US
Steven Lee - Doylestown PA, US
David Roush - Colts Neck NJ, US

C07K014/775

530/359000

There is disclosed a process for purifying a natural product using a two-phase, multi-solvent system followed by vacuum concentration and back extraction. The method allows for the removal of impurities by controlling the polarity balance of a two-phase system by manipulating the proportions of the four solvents and subsequently the relative distribution of the product versus the impurities.

20120165513, Jun 28, 2012

Aug 30, 2010

13/393638

Jennifer M. Pollard - Monroe NJ, US
Rebecca A. Chmielowski - Clark NJ, US
Lisa M. Dietrich - Granville OH, US
Francis P. Gailliot - Cranford NJ, US
Kent Goklen - West Conshohocken PA, US

C07H 15/26

536 181

The present disclosure relates to processes useful in the isolation and purification of enfumafungin, which is classified as a triterpene glycoside antifungal compound and acts as a glucan synthase inhibitor. Enfumafungin has application in the treatment of conditions caused by fungal infection and is also useful as an intermediate in the preparation of other compounds useful as antifungal agents and/or inhibitors of (1,3)-β-D-glucan synthesis.

20140018525, Jan 16, 2014

Mar 29, 2012

14/007689

Kent E. Goklen - King of Prussia PA, US
Eric J. Suda - Phoenixville PA, US
Antonio Raul Ubiera - King of Prussia PA, US

GLAXOSMITHKLINE LLC - Philadelphia PA

C07K 1/18

530416, 2524081

The invention is directed to a method for producing a polypeptide composition comprising: combining a polypeptide with a volatile additive to form a liquid mixture and lyophilizing the liquid mixture to obtain a lyophilized polypeptide composition.

20230046182, Feb 16, 2023

Nov 1, 2022

17/978791

- Brentford, GB
Kent E. GOKLEN - King of Prussia PA, US
Nicholas E. LEVY - King of Prussia PA, US
Jessica Rachel - King of Prussia PA, US
Andrew S. THOMSON - Collegeville PA, US
Kenneth G. YANCEY - Wayne PA, US

C07K 1/22
B01D 15/38
B01D 15/42
C07K 16/06

The present invention relates to a method of purifying a recombinant polypeptide from Host Cell Proteins (HCP), the method comprising: (a) applying a solution comprising the recombinant polypeptide and HCP to a superantigen chromatography solid support, (b) washing the superantigen chromatography solid support with a wash buffer comprising caprylate and arginine; and (c) eluting the recombinant polypeptide from the superantigen chromatography solid support.

20210284686, Sep 16, 2021

Sep 6, 2017

16/330588

- Brentford, Middlesex, GB
Kent E GOKLEN - King of Prussia PA, US
Nicolas E LEVY - King of Prussia PA, US
Jessica Rachel MOLEK - King of Prussia PA, US
Andrew S. THOMSON - Collegeville PA, US
Kenneth G. YANCY - Wayne PA, US

C07K 1/22
C07K 16/06
B01D 15/38
B01D 15/42

The present invention relates to a method of purifying a recombinant polypeptide from Host Cell Proteins (HCP), the method comprising: (a) applying a solution comprising the recombinant polypeptide and HCP to a superantigen chromatography solid support, (b) washing the superantigen chromatography solid support with a wash buffer comprising caprylate and arginine; and (c) eluting the recombinant polypeptide from the superantigen chromatography solid support.

20210054051, Feb 25, 2021

Mar 6, 2019

16/977142

- Brentford Middlesex, GB
Kent E. GOKLEN - Collegeville PA, US
Nicholas E. LEVY - Collegeville PA, US
Jessica Rachel MOLEK - Collegeville PA, US
Andrew S. THOMSON - Collegeville PA, US
Kenneth G. YANCEY - Ithaca NY, US

C07K 16/06
C07K 16/28

A method of purifying a recombinant polypeptide from a Host Cell Protein (HCP), wherein the amino acid sequence of the recombinant polypeptide comprises a cathepsin L cleavage site is provided. The method includes: (a) applying a solution containing the recombinant polypeptide and HCP to a superantigen chromatography solid support, (b) washing the superantigen chromatography solid support with a wash buffer containing caprylate and arginine; and (c) eluting the recombinant polypeptide from the superantigen chromatography solid support. A method of purifying an anti-OX40 antigen binding polypeptide from a Host Cell Protein (HCP) is also provided.