Inventors:
- Santa Clara CA, US
- Philadelphia PA, US
Jifen LI - Philadelphia PA, US
Stephen FISHER - Philadelphia PA, US
Youtao LU - Philadelphia PA, US
Junhyong KIM - Philadelphia PA, US
Jinchun WANG - Santa Clara CA, US
Mimi HEALY - Santa Clara CA, US
Assignee:
Agilent Technologies, Inc. - Santa Clara CA
The Trustees of the University of Pennsylvania - Philadelphia PA
International Classification:
C12N 15/11
C12Q 1/6816
C07H 19/10
Abstract:
Provided herein are compositions and methods to assess the genomic landscape of fixed cells using light activated oligonucleotides that can be directed to the nucleus, mitochondria, or cytoplasm of fixed cells and that, upon activation, can be extended for in situ copying of nuclear single-stranded DNA (i.e., open chromatin), open mitochondrial DNA, and/or cytoplasmic RNA into barcoded complementary DNA. These methods also provide for gene specific 3D chromatin structural niche analysis.