Jun S Lee

53818121, Jan 17, 1995

May 21, 1993

8/065433

Jun Lee - Flushing NY

A45B 2528

135 48

An inexpensive rain water collecting device which is easy to manufacture. The rain water collecting device provides an effective device which is adaptable to various umbrella tip sizes and avoids any bacterial growth problems. The rain water collecting device includes at least a reservoir having a first open end and a second open end, the first open end has an opening with an inner perimeter which is substantially equal to an outer perimeter of the umbrella tip, and the second open end has an opening with a perimeter which is greater than the inner perimeter of the opening of the first open end; a ferrule formed about the opening of the first open end; and at least one flow barrier about the second open end of the reservoir. After using an umbrella in the rain, it is collapsed. The rain water on the cloth of the umbrella then drains into the reservoir of the rain water collecting device.

20160215281, Jul 28, 2016

Jan 21, 2016

15/003179

- Carlsbad CA, US
Craig BRANCH - Oceanside CA, US
Jun LEE - San Diego CA, US
Todd Peterson - Coronado CA, US
Karl HECKER - San Diego CA, US

C12N 15/10
C12Q 1/68

Disclosed are rapid and reproducible methods for separating and purifying nucleic acids from paraffin-containing tissue samples. The disclosed methods involve a melting step, where paraffin-containing tissue samples are heated in the presence of a detergent, causing the paraffin to melt and tissue sample cells to lyse. From the resulting two-phase mixture (i.e., a paraffin phase and an aqueous phase), the aqueous phase is collected for purification of nucleic acid. Protease(s) can be used at one or more points in the separation process to facilitate tissue cell lysis and/or degrade proteins that could degrade nucleic acid or interfere with subsequent genetic manipulation or analysis.