Search

Joshua Mosberg Phones & Addresses

  • New York, NY
  • Boston, MA
  • Cambridge, MA
  • Ann Arbor, MI

Publications

Us Patents

Methods Of Introducing Nucleic Acids Into Cellular Dna

View page
US Patent:
20140045267, Feb 13, 2014
Filed:
Jul 30, 2013
Appl. No.:
13/954351
Inventors:
Marc J. LAJOIE - Cambridge MA, US
Christopher J. GREGG - Roslindale MA, US
Joshua A. MOSBERG - Cambridge MA, US
George M. CHURCH - Brookline MA, US
Assignee:
PRESIDENT AND FELLOWS OF HARVARD COLLEGE - Cambridge MA
International Classification:
C12N 15/70
US Classification:
435471
Abstract:
A method of introducing a nucleic acid sequence into a cell is provided where the cell has impaired or inhibited or disrupted primase activity or impaired or inhibited or disrupted helicase activity, or larger or increased gaps or distance between Okazaki fragments or lowered or reduced frequency of Okazaki fragment initiation, or the cell has increased single stranded DNA (ssDNA) on the lagging strand of the replication fork including transforming the cell through recombination with a nucleic acid oligomer.The present invention relates to methods of introducing one or more nucleic acid sequences into a cell having impaired or inhibited or disrupted primase activity or impaired or inhibited or disrupted helicase activity, or larger or increased gaps or distance between Okazaki fragments or lowered or reduced frequency of Okazaki fragment initiation.

Methods Of Introducing Nucleic Acids Into Cellular Dna

View page
US Patent:
20170183666, Jun 29, 2017
Filed:
Feb 7, 2017
Appl. No.:
15/426527
Inventors:
- Cambridge MA, US
Christopher J. Gregg - Roslindale MA, US
Joshua A. Mosberg - Cambridge MA, US
George M. Church - Brookline MA, US
International Classification:
C12N 15/72
C12N 15/10
Abstract:
A method of introducing a nucleic acid sequence into a cell is provided where the cell has impaired or inhibited or disrupted primase activity or impaired or inhibited or disrupted helicase activity, or larger or increased gaps or distance between Okazaki fragments or lowered or reduced frequency of Okazaki fragment initiation, or the cell has increased single stranded DNA (ssDNA) on the lagging strand of the replication fork including transforming the cell through recombination with a nucleic acid oligomer.
Joshua A Mosberg from New York, NY, age ~37 Get Report