US Patent:
20020137161, Sep 26, 2002
Inventors:
Dietmar Manstein - Heidelberg, DE
Jon Kull - Enfield NH, US
Menno Knetsch - Maastricht, NL
Hartmut Niemann - Heidelberg, DE
Assignee:
Max-Planck-Gesellschaft zur Forderung der Wissenschaftern e.V. - Munchen
International Classification:
C12N009/00
C07H021/04
C12P021/02
C12N005/06
US Classification:
435/183000, 435/069100, 435/325000, 435/320100, 536/023200
Abstract:
A recombinant protein comprised of an amino acid sequence of a motor protein, a target protein of interest, and optionally, a linker sequence between the two proteins, is disclosed. Also disclosed are a DNA sequence encoding such a recombinant protein, a vector expressing such a recombinant protein, a host cell transformed with such a vector, a method for producing such a recombinant protein, and methods for purification, crystallization and structure elucidation of such a recombinant protein. In preferred embodiments of the invention, the motor protein is a member of the kinesin or myosin superfamilies, or an analog, fragment or derivative thereof.