Inventors:
Deane F. Mosher - Madison WI
Jane M. Sottile - Madison WI
Assignee:
Wisconsin Alumni Research Foundation - Madison WI
International Classification:
C07K 122
C07K 114
C12N 1509
C12N 1563
Abstract:
Recombinant protein purification vectors and methods for their use are disclosed. The vectors contain a DNA sequence coding for a gelatin binding region of fibronectin. The vectors express a foreign DNA sequence of interest fused to the fibronectin portion. Secretion signals on the fused product assist the product in being secreted from a production cell. The product can then be purified on a gelatin-containing affinity column and digested with a protease, such as trypsin, to cleave the desired protein from the gelatin binding region. The protein then contains no more than one extraneous amino acid.