Jane M Rachel from 28 W 69Th St, Kansas City, MO 64113, age 73, Phone: (816) 363-9239

Resumes

Director, Education And Scientific Development; Manager, Molecular And Flow Cytometry

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Location:

Kansas City, MO

Industry:

Hospital & Health Care

Work:

Saint Luke's Health System
Director, Education and Scientific Development; Manager, Molecular and Flow Cytometry

Director, Education And Scientific Development

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Location:

Kansas City, MO

Industry:

Hospital & Health Care

Work:

Saint Lukes Hospital
Director, Education and Scientific Development

Education:

University of Missouri - Kansas City
Bachelors University of Kansas Medical Center
Masters, Immunology

Skills:

Healthcare
Clinical Research
Clinical Trials
Team Leadership
Contract Management
Teaching
Public Speaking
Research
Healthcare Management

Publications

Us Patents

Solid Phase Indicator Red Blood Cells And Method

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US Patent:

48164135, Mar 28, 1989

Filed:

Sep 8, 1986

Appl. No.:

6/904948

Inventors:

Lyle T. Sinor - Merriam KS
Frederick V. Plapp - Overland Park KS
Jane M. Rachel - Kansas City MO

Assignee:

Immucor, Inc. - Norcross GA

International Classification:

G01N 33555
G01N 33549

US Classification:

436520

Abstract:

A method and composition for use in preparing red blood cells for use as indicator cells to detect the presence of antigens and antibodies in biological fluids is the subject of the present invention. Freshly collected human or animal erythrocytes are washed six times with isotonic saline, and suspended to a hematocrit of four percent (volume/volume) with isotonic saline. An incomplete antibody, specific for the antigen or antibody to be detected, in a quantity less than that which would cause irreversible agglutination, is added to the erythrocyte solution. The mixture is incubated, washed, and suspended to a hematocrit of 3-5 (v/v) with isotonic saline. A second antibody, immuno specific for the first antibody and in an amount sufficiently great to immunologically bind substantially all of the antibody carrying red blood cells but not so great as to cause irreversible agglutination, is attached to the erythrocytes. This mixture is incubated, washed, and suspended to a hematocrit of 0. 1-1% (v/v) in a media containing red blood cell nutrients.

Assay Method For Gamma Glutamyltransferase (Ggt) In Liquid Blood And Dried Blood

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US Patent:

50968122, Mar 17, 1992

Filed:

Jun 8, 1989

Appl. No.:

7/363216

Inventors:

Jane M. Rachel - Kansas City MO
Laura M. Smith - Kansas City MO
Linda R. Pfaltzgraff - Osawatomie KS

Assignee:

Osborn Laboratories, Inc. - Shawnee Mission KS

International Classification:

C12Q 148
C12Q 137

US Classification:

435 15

Abstract:

A method of gamma glutamyltransferase (GGT) analysis is suitable for use with hemolyzed fluid or dried whole blood samples which avoids the need for protein precipitation or centrifugation of the sample. Analysis is by first mixing a fluid or dried whole blood sample with a phosphate buffered saline solution containing a non-ionic biological detergent. The mixture is then vigorously agitated for one hour at room temperature. The activity of the GGT is then determined by reacting the mixture with a GGT substrate. Color is developed by a diazo coupling reaction with the enzyme reaction product. The intensity of the color is then measured.

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