Jaeseok Han Phones & Addresses
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44714 Corte Sanchez, Temecula, CA 92592 (951) 331-2094
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San Diego, CA
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Ann Arbor, MI
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Lake Bluff, IL
Publications
Us Patents
Glucose Inducible Insulin Expression And Methods Of Treating Diabetes
View pageUS Patent:
20070020237, Jan 25, 2007
Filed:
May 30, 2006
Appl. No.:
11/443575
Inventors:
Chungja Yoon - Rockville MD, US
Ji-Won Yoon - Rockville MD, US
Jaeseok Han - Lake Bluff IL, US
Ji-Won Yoon - Rockville MD, US
Jaeseok Han - Lake Bluff IL, US
International Classification:
C12N 5/08
A61K 48/00
C12N 15/86
A61K 48/00
C12N 15/86
US Classification:
424093200, 435456000, 435366000
Abstract:
The invention provides an isolated tissue specific glucose responsive promoter having a polymerase binding domain 3′ to at least one tripartite transcription factor binding cis element having a hepatocyte nuclear factor-1 (HNF-1) element, a CAAT/enhancer binding protein (C/EBP) response element and a glucose-response element (GRE). The promoter can include a second or third tripartite transcription factor binding cis element. A host cell including the tissue specific glucose responsive promoter of the invention also are provided. Further provided is a method of treating or preventing diabetes. The method includes administering to an individual an effective amount of a viral particle having a vector comprising a tissue specific glucose responsive promoter comprising a polymerase binding domain 3′ to at least one tripartite transcription factor binding cis element having a hepatocyte nuclear factor-1 (HNF-1) element, a CAAT/enhancer binding protein (C/EBP) response element and a glucose-response element (GRE) operationally linked to an insulin encoding nucleic acid, wherein expression of the insulin encoding nucleic acid is tissue specific and glucose responsive.