Inventors:
Arnold R. Castro - Monroe GA, US
Huiying Wang - Lake Oswego OR, US
Assignee:
The United States of America as represented by the Department of Health and Human Services, Centers for Disease Control and Prevention - Washington DC
International Classification:
G01N 33/53
G01N 33/573
Abstract:
Compositions, methods and devices for the detection of anti-lipoidal antibodies and the diagnosis of disease, for example, syphilis, are described. In particular, oxidized cardiolipins, which may be conjugated with a variety of attachment molecules, such as BSA, KLH, biotin, synthetic protein MAPS, IgY, streptavidin, or avidin, are described. Such oxidized cardiolipin, alone or complexed with one or more attachment molecules, are useful to detect anti-lipoidal antibodies in subjects, for example, when used in lateral flow devices. Lateral flow devices are described that permit the detection of anti-lipoidal antibodies and that permit the co-detection of nontreponemal and treponemal antibodies in biological samples.