George N Havrilla

6858148, Feb 22, 2005

Jul 16, 2003

10/621825

Benjamin P. Warner - Los Alamos NM, US
George J. Havrilla - Los Alamos NM, US
Thomasin C. Miller - Los Alamos NM, US
Cyndi A. Wells - Los Alamos NM, US

The Regents of the University of California - Los Alamos NM

B01D015/08

210656, 2101982, 436 56, 436 58, 435 71, 378 46

The method for screening binding between a target binder and potential pharmaceutical chemicals involves sending a solution (preferably an aqueous solution) of the target binder through a conduit to a size exclusion filter, the target binder being too large to pass through the size exclusion filter, and then sending a solution of one or more potential pharmaceutical chemicals (preferably an aqueous solution) through the same conduit to the size exclusion filter after target binder has collected on the filter. The potential pharmaceutical chemicals are small enough to pass through the filter. Afterwards, x-rays are sent from an x-ray source to the size exclusion filter, and if the potential pharmaceutical chemicals form a complex with the target binder, the complex produces an x-ray fluorescence signal having an intensity that indicates that a complex has formed.

7241381, Jul 10, 2007

Nov 10, 2004

10/986519

Benjamin P. Warner - Los Alamos NM, US
George J. Havrilla - Los Alamos NM, US
Thomasin C. Miller - Los Alamos NM, US
Cyndi A. Wells - Los Alamos NM, US

Los Alamos National Security, LLC - Los Alamos NM

B01D 15/08

2101982, 210656, 378 47, 422 8208, 435 71, 436 56, 436 58

The method for screening binding between a target binder and potential pharmaceutical chemicals involves sending a solution (preferably an aqueous solution) of the target binder through a conduit to a size exclusion filter, the target binder being too large to pass through the size exclusion filter, and then sending a solution of one or more potential pharmaceutical chemicals (preferably an aqueous solution) through the same conduit to the size exclusion filter after target binder has collected on the filter. The potential pharmaceutical chemicals are small enough to pass through the filter. Afterwards, x-rays are sent from an x-ray source to the size exclusion filter, and if the potential pharmaceutical chemicals form a complex with the target binder, the complex produces an x-ray fluorescence signal having an intensity that indicates that a complex has formed.

7519145, Apr 14, 2009

May 31, 2006

11/444660

Benjamin P. Warner - Los Alamos NM, US
George J. Havrilla - Los Alamos NM, US
Thomasin C. Miller - Bartlesville OK, US
Cris Lewis - Los Alamos NM, US
Cynthia A. Mahan - Los Alamos NM, US
Cyndi A. Wells - Los Alamos NM, US

Los Alamos National Security, LLC - Los Alamos NM

G01N 23/223

378 47

Method and apparatus for screening chemicals using micro x-ray fluorescence. A method for screening a mixture of potential pharmaceutical chemicals for binding to at least one target binder involves flow-separating a solution of chemicals and target binders into separated components, exposing them to an x-ray excitation beam, detecting x-ray fluorescence signals from the components, and determining from the signals whether or not a binding event between a chemical and target binder has occurred.

7858385, Dec 28, 2010

May 16, 2001

09/859701

Benjamin P. Warner - Los Alamos NM, US
George J. Havrilla - Los Alamos NM, US
Grace Mann - Hong Kong, HK

Los Alamos National Security, LLC - Los Alamos NM

G01N 21/76
G01N 33/543
G01N 33/00

436172, 436518, 436103, 436119, 436124, 430966

Method for detecting binding events using micro-X-ray fluorescence spectrometry. Receptors are exposed to at least one potential binder and arrayed on a substrate support. Each member of the array is exposed to X-ray radiation. The magnitude of a detectable X-ray fluorescence signal for at least one element can be used to determine whether a binding event between a binder and a receptor has occurred, and can provide information related to the extent of binding between the binder and receptor.

7929662, Apr 19, 2011

Mar 3, 2009

12/396592

Benjamin P. Warner - Los Alamos NM, US
George J. Havrilla - Los Alamos NM, US
Thomasin C. Miller - Bartlesville OK, US
Cris Lewis - Los Alamos NM, US
Cynthia A. Mahan - Los Alamos NM, US
Cyndi A. Wells - Los Alamos NM, US

Los Alamos National Security, LLC - Los Alamos NM

G01N 23/223

378 47

Method and apparatus for screening chemicals using micro x-ray fluorescence. A method for screening a mixture of potential pharmaceutical chemicals for binding to at least one target binder involves flow separating a solution of chemicals and target binders into separated components, exposing them to an x-ray excitation beam, detecting x-ray fluorescence signals from the components, and determining from the signals whether or not a binding event between a chemical and target binder has occurred.

20050214847, Sep 29, 2005

May 9, 2005

11/125036

George Havrilla - Los Alamos NM, US
Thomasin Miller - Los Alamos NM, US
Benjamin Warner - Los Alamos NM, US
Cris Lewis - Los Alamos NM, US
Cynthia Mahan - Los Alamos NM, US
Cyndi Wells - Los Alamos NM, US

C12Q001/68
G01N033/53
C12M001/34

435006000, 435007100, 435287200, 378001000

Flow method and apparatus for screening chemicals using micro x-ray fluorescence. The invention includes a method and apparatus for screening a mixture of potential pharmaceutical chemicals for binding to at least one target binder. According to the method, after preparing a solution of potential pharmaceutical chemicals with at least one target binder, the solution is flow-separated into at least two separated components. Each component is exposed to an x-ray excitation beam. Any component that emits a detectable x-ray fluorescence signal is isolated.

20070170362, Jul 26, 2007

Jan 25, 2006

11/339045

Brian Patterson - Los Alamos NM, US
George Havrilla - Los Alamos NM, US

G01J 5/02

250339070

An internal reflectance element assembly, that is defined by a crystal that has at least one measured dimension of about 5 mm or greater that is held in a holder structure shaped to hold the crystal, and a backer plate to secure a sample to the crystal.