Fabian J Lionetti

40049752, Jan 25, 1977

Dec 30, 1975

5/645161

Fabian J. Lionetti - Milton MA
Stephen M. Hunt - Allston MA

The United States of America as represented by the Secretary of the Navy - Washington DC

C12K 900
F25D 2500
A61K 3514

195 18

A method of salvaging granulocytes, or white cells, from buffy coat of ceifuged whole blood is provided. The sequence of operations leading to the separation red cells, white cells, platelets and plasma is presented in a flow diagram of a bag system for the cryopreservation of leukocytes, with freezing of the white cells accomplished by the introduction of a combination of hydroxyethyl starch (HES), which functions as both a sedimenting agent and a cryoprotective agent, and dimethylsulfoxide (DMSO), a cryoprotective agent. A preferred combination is 4% HES with 5% DMSO. White cell separation is interfaced with collection methods for plasma and platelets so as to conserve all major cell types.

40189112, Apr 19, 1977

Nov 10, 1975

5/630558

Fabian J. Lionetti - Milton MA
Stephen M. Hunt - Allston MA

The United States of America as represented by the Secretary of the Navy - Washington DC

A61K 3518
F25C 118
C12K 900

424101

A rigid metal holder having perforated side plates for containing plastic gs of human red blood cells for both freezing and thawing such cells is provided. Hydroxyethylstarch, HES, is used as the cryoprotective agent and, together with the perforated metal plates, permits the very fast freezing rates required to produce viable red cells. The HES is transfusable with the thawed cells so that post-thaw washing is obviated.