Inventors:
- Ipswich MA, US
Brittany S. Sexton - Amesbury MA, US
Margaret Heider - Ipswich MA, US
Louise JS Williams - Reading MA, US
Eileen T. Dimalanta - Wakefield MA, US
Assignee:
New England Biolabs, Inc. - Ipswich MA
International Classification:
C12Q 1/6869
C12N 15/10
C12Q 1/6855
Abstract:
Provided herein is a polymerase-free enzyme mix (FRAG) for fragmenting double-stranded DNA. In some embodiments the enzyme mix may comprise a double-stranded DNA nickase and at least one of a DNA ligase capable of sealing a nick within a DNA, and a single-strand specific DNA nuclease. Methods for fragmenting double-stranded DNA are also provided.