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Dorothea K Torous

from Rochester, NY
Age ~62

Dorothea Torous Phones & Addresses

  • 181 Cornwall Ln, Rochester, NY 14617 (585) 467-5448
  • Irondequoit, NY
  • Yonkers, NY
  • 181 Cornwall Ln, Rochester, NY 14617 (585) 943-3358

Work

Position: Food Preparation and Serving Related Occupations

Education

Degree: Associate degree or higher

Emails

d***s@lycos.com

Business Records

Name / Title
Company / Classification
Phones & Addresses
Dorothea Torous
Director Of Testing
Litron Laboratories LTD
Biotechnology · Testing Laboratories
200 Canal Vw Blvd #106, Rochester, NY 14623
(585) 442-0930, (585) 442-0934

Publications

Us Patents

Method For Enumerating Mammalian Cell Micronuclei With An Emphasis On Differentially Staining Micronuclei And The Chromatin Of Dead And Dying Cells

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US Patent:
7445910, Nov 4, 2008
Filed:
Jun 24, 2005
Appl. No.:
11/166433
Inventors:
Stephen D. Dertinger - Webster NY, US
Siân E. Cairns - Clifton Park NY, US
Svetlana L. Avlasevich - Webster NY, US
Dorothea K. Torous - Rochester NY, US
Assignee:
Litron Laboratories, Ltd. - Rochester NY
International Classification:
G01N 1/30
US Classification:
435 405
Abstract:
The present invention relates a method for the enumeration of mammalian cell micronuclei, while distinguishing micronuclei from the chromatin of dead and dying cells. The method utilizes differential staining of chromatin from dead and dying cells, to distinguish the chromatin from micronuclei and nuclei that can be detected based upon fluorescent emission and light scatter following exposure to an excitatory light source. Counting of micronuclei events relative to the number of nuclei can be used to assess the DNA-damaging potential of a chemical agent, the DNA-damaging potential of a physical agent, the effects of an agent which can modify endogenously-induced DNA damage, and the effects of an agent which can modify exogenously-induced DNA damage. Kits for practicing the invention are also disclosed.

Kit For Enumerating Mammalian Cell Micronuclei

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US Patent:
7645593, Jan 12, 2010
Filed:
Oct 2, 2008
Appl. No.:
12/244179
Inventors:
Stephen D. Dertinger - Webster NY, US
Sian E. Cairns - Clifton Park NY, US
Svetlana L. Avlasevich - Webster NY, US
Dorothea K. Torous - Rochester NY, US
Assignee:
Litron Laboratories, Ltd. - Rochester NY
International Classification:
C12Q 1/02
G01N 1/30
G01N 33/48
US Classification:
435 29, 435 405, 435259
Abstract:
The present invention relates a method for the enumeration of mammalian cell micronuclei, while distinguishing micronuclei from the chromatin of dead and dying cells. The method utilizes differential staining of chromatin from dead and dying cells, to distinguish the chromatin from micronuclei and nuclei that can be detected based upon fluorescent emission and light scatter following exposure to an excitatory light source. Counting of micronuclei events relative to the number of nuclei can be used to assess the DNA-damaging potential of a chemical agent, the DNA-damaging potential of a physical agent, the effects of an agent which can modify endogenously-induced DNA damage, and the effects of an agent which can modify exogenously-induced DNA damage. Kits for practicing the invention are also disclosed.

Method For Enumeration Of Mammalian Cell Micronuclei

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US Patent:
8524449, Sep 3, 2013
Filed:
Jan 8, 2010
Appl. No.:
12/684243
Inventors:
Stephen D. Dertinger - Webster NY, US
Sian E. Cairns - Clifton Park NY, US
Svetlana L. Avlasevich - Webster NY, US
Dorothea K. Torous - Rochester NY, US
Assignee:
Litron Laboratories, Ltd. - Rochester NY
International Classification:
C12Q 1/68
C12Q 1/02
G01N 1/30
C12N 1/06
US Classification:
435 61, 435 29, 435 405, 435259
Abstract:
The present invention relates a method for the enumeration of mammalian cell micronuclei, while distinguishing micronuclei from the chromatin of dead and dying cells. The method utilizes differential staining of chromatin from dead and dying cells, to distinguish the chromatin from micronuclei and nuclei that can be detected based upon fluorescent emission and light scatter following exposure to an excitatory light source. Counting of micronuclei events relative to the number of nuclei can be used to assess the DNA-damaging potential of a chemical agent, the DNA-damaging potential of a physical agent, the effects of an agent which can modify endogenously-induced DNA damage, and the effects of an agent which can modify exogenously-induced DNA damage. Kits for practicing the invention are also disclosed.

Method For The Enumeration Of Mammalian Micronucleated Erythrocyte Populations With A Single-Laser Flow Cytometer

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US Patent:
20030134305, Jul 17, 2003
Filed:
Aug 16, 2002
Appl. No.:
10/223305
Inventors:
Stephen Dertinger - Webster NY, US
Dorothea Torous - Rochester NY, US
Carol Tometsko - Rochester NY, US
International Classification:
C12Q001/68
G01N033/567
G06F019/00
G01N033/48
G01N033/50
US Classification:
435/006000, 435/007210, 702/021000
Abstract:
A single-laser flow cytometric method for the enumeration of micronucleated erythrocyte populations is disclosed which affords superior fluorescent resolution of young reticulocytes from normochromatic erythrocytes, particularly in mammals that exhibit efficient splenic sequestering. The method can also be used for assessing the DNA damaging potential of pharmaceuticals undergoing clinical trials; evaluating patient-specific responses to chemotherapy or radiation therapy; evaluating compounds, diets, or other factors that may protect against DNA damage resulting from endogenous or exogenous agents; evaluating compounds, diets, or other factors that may potentiate DNA damage resulting from endogenous or exogenous agents; determining the level of DNA damage in a population following a major accident; and monitoring workers who may be occupationally exposed to DNA damaging agents

Method For Enumeration Of Mammalian Cell Micronuclei, While Distinguishing Chromatin Of Dead And Dying Cells

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US Patent:
20130337465, Dec 19, 2013
Filed:
Aug 16, 2013
Appl. No.:
13/969185
Inventors:
Sian E. CAIRNS - Clifton Park NY, US
Svetlana L. AVLASEVICH - Webster NY, US
Dorothea K. TOROUS - Rochester NY, US
Assignee:
Litron Laboratories, Ltd. - Rochester NY
International Classification:
C12Q 1/68
US Classification:
435 619
Abstract:
The present invention relates a method for the enumeration of mammalian cell micronuclei, while distinguishing micronuclei from the chromatin of dead and dying cells. The method utilizes differential staining of chromatin from dead and dying cells, to distinguish the chromatin from micronuclei and nuclei that can be detected based upon fluorescent emission and light scatter following exposure to an excitatory light source. Counting of micronuclei events relative to the number of nuclei can be used to assess the DNA-damaging potential of a chemical agent, the DNA-damaging potential of a physical agent, the effects of an agent which can modify endogenously-induced DNA damage, and the effects of an agent which can modify exogenously-induced DNA damage. Kits for practicing the invention are also disclosed.

Method For The Enumeration Of Micronucleated Erythrocyte Populations With A Single Laser Flow Cytometer

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US Patent:
58586679, Jan 12, 1999
Filed:
Sep 6, 1996
Appl. No.:
8/706680
Inventors:
Stephen Dertinger - Webster NY
Dorothea Torous - Rochester NY
Kenneth Tometsko - Rochester NY
Assignee:
Litron Laboratories - Rochester NY
International Classification:
C12Q 168
G01N 3353
C07H 2102
C12N 1500
US Classification:
435 6
Abstract:
A single laser flow cytometric method for the enumeration of micronuclei in erythrocyte populations, wherein a sample of peripheral blood or bone marrow is obtained and the cell populations in the sample are fixed. Reticulocytes in the fixed samples are treated simultaneously with RNAse and with a fluorescent labeled antibody having binding specificity for a surface marker for erythroblasts/reticulocytes. The erythrocyte populations are then stained with a nucleic acid stain which stains DNA representing micronuclei, if present. The stained and/or labelled erythrocyte populations are then exposed to a laser beam of appropriate excitation wavelength for both the nucleic acid staining dye and the fluorescent label to produce fluorescent emission. The fluorescent emission and light scatter produced by the erythrocyte populations are detected by the flow cytometer from which is calculated the number of specific erythrocyte populations in said sample.

Method For The Enumeration Of Micronucleated Erythrocyte Populations With A Single Laser Flow Cytometer

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US Patent:
61000388, Aug 8, 2000
Filed:
Jan 11, 1999
Appl. No.:
9/228329
Inventors:
Stephen D. Dertinger - Webster NY
Dorothea K. Torous - Rochester NY
Kenneth R. Tometsko - Rochester NY
Assignee:
Litron Laboratories Limited - Rochester NY
International Classification:
C12Q 168
G01N 3353
G01N 33566
G01N 33563
C07K 1600
US Classification:
435 6
Abstract:
A single laser flow cytometric method for the enumeration of micronuclei in erythrocyte populations, wherein a sample of peripheral blood or bone marrow is obtained and the cell populations in the sample are fixed. Reticulocytes in the fixed samples are treated simultaneously with RNAse and with a fluorescent labeled antibody having binding specificity for a surface marker for erythroblasts/reticulocytes. The erythrocyte populations are then stained with a nucleic acid stain which stains DNA representing micronuclei, if present. The stained and/or labeled erythrocyte populations are then exposed to a laser beam of appropriate excitation wavelength for both the nucleic acid staining dye and the fluorescent label to produce fluorescent emission. The fluorescent emission and light scatter produced by the erythrocyte populations are detected by the flow cytometer from which is calculated the number of specific erythrocyte populations in said sample.

Method And Composition For Lyophilizing Red Blood Cells

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US Patent:
57503303, May 12, 1998
Filed:
Jun 19, 1996
Appl. No.:
8/666134
Inventors:
Andrew M. Tometsko - late of Rochester NY
Stephen Dertinger - Webster NY
Dorothea Torous - Rochester NY
Kenneth Tometsko - Rochester NY
Assignee:
Litron Laboratories - Rochester NY
International Classification:
A01N 102
US Classification:
435 2
Abstract:
Disclosed are a composition for the lyophilization of mammalian red blood cells comprising a hydrophilic polymer, a carbohydrate, and an organic solvent; and a method of using the composition to lyophilize red blood cells comprising mixing red blood cells with the composition, freezing the mixture, and drying the mixture by removing water by sublimation. Also disclosed are red blood cells lyophilized according to this method for lyophilization, and a method for reconstituting the lyophilized red blood cells. In particular, the composition used to lyophilize the red blood cells comprises a mixture of a hydrophilic polymer ranging from 1,450-20,000 Daltons at 5-50% w/v, a mono- or disaccharide or a mixture thereof from 0. 01-0. 2M and an organic solvent such as a primary alcohol, a secondary alcohol, dimethyl sulfoxide or combinations thereof at 0. 5-20% v/v.
Dorothea K Torous from Rochester, NY, age ~62 Get Report