Resumes
Resumes
Chudi Guan Phones & Addresses
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10 Great Pond Rd, Wenham, MA 01984 (978) 468-5269
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Allen, TX
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Beverly, MA
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Colton, TX
Work
Position:
Construction and Extraction Occupations
Education
Degree:
Bachelor's degree or higher
Emails
Publications
Us Patents
Isolation And Composition Of Novel Glycosidases
View pageUS Patent:
6342365, Jan 29, 2002
Filed:
Feb 24, 1999
Appl. No.:
09/257153
Inventors:
Sharon T. Wong-Madden - Bellevue WA
Ellen P. Guthrie - Andover MA
David Landry - Essex MA
Christopher H. Taron - Champaign IL
Chudi Guan - Wenham MA
Phillips W. Robbins - Acton MA
Ellen P. Guthrie - Andover MA
David Landry - Essex MA
Christopher H. Taron - Champaign IL
Chudi Guan - Wenham MA
Phillips W. Robbins - Acton MA
Assignee:
New England Biolabs, Inc. - Beverly MA
International Classification:
C12Q 134
US Classification:
435 18, 435 4, 435 74, 435 76, 435 772, 435183, 435200, 435201, 435207, 435208
Abstract:
Substantially pure glycosidases capable for cleaving selected glycosidic bonds have been described including glycosidases isolated from Xanthomonas and recombinant glycosidases. Substrate specificity of isolated enzymes have been identified for GlcNac1-X, Gal1-3R, Gal1-6R, Gal1-3R, Fuc1-2R, Fuc1-3R, Fuc1-4R, Man1-2R, Man1-3R, Man1-6R, Man1-4R, Xyl1-2R, Glc1-4R, and Gal1-4R providing improved capability for selectively cleaving a glycosidic linkage in a carbohydrate substrate and for forming modified carbohydrates.
Isolation And Composition Of Novel Glycosidases
View pageUS Patent:
6358724, Mar 19, 2002
Filed:
Jun 18, 2001
Appl. No.:
09/883800
Inventors:
Sharon T. Wong-Madden - Bellevue WA
Ellen P. Guthrie - Andover MA
David Landry - Essex MA
Christopher H. Taron - Champaign IL
Chudi Guan - Wenham MA
Phillips W. Robbins - Acton MA
Ellen P. Guthrie - Andover MA
David Landry - Essex MA
Christopher H. Taron - Champaign IL
Chudi Guan - Wenham MA
Phillips W. Robbins - Acton MA
Assignee:
New England Biolabs, Inc. - Beverly MA
International Classification:
C12N 938
US Classification:
435207, 435 4, 435 18, 435200, 435243, 4352521
Abstract:
Substantially pure glycosidases capable for cleaving selected glycosidic bonds have been described including glycosidases isolated from Xanthomonas and recombinant glycosidases. Substrate specificity of isolated enzymes have been identified for GlcNac1-X, Gal1-3R, Gal1-6R, Gal1-3R, Fuc-2R, Fuc1-3R, Fuc1-4R, Man1-2R, Man1-3R, Man1-6R, Man1-4R, Xyl1-2R, Glc1-4R, and Gal1-4R providing improved capability for selectively cleaving a glycosidic linkage in a carbohydrate substrate and for forming modified carbohydrates.
Isolation And Composition Of Novel Glycosidases
View pageUS Patent:
7094563, Aug 22, 2006
Filed:
Nov 15, 2001
Appl. No.:
10/003136
Inventors:
Sharon T. Wong-Madden - Bellevue WA, US
Ellen P. Guthrie - Andover MA, US
David Landry - Essex MA, US
Christopher H. Taron - Champaign IL, US
Chudi Guan - Wenham MA, US
Phillips W. Robbins - Acton MA, US
Ellen P. Guthrie - Andover MA, US
David Landry - Essex MA, US
Christopher H. Taron - Champaign IL, US
Chudi Guan - Wenham MA, US
Phillips W. Robbins - Acton MA, US
Assignee:
New England Biolabs, Inc. - Ispwich MA
International Classification:
C12P 1/00
C12P 19/22
C12N 9/26
C12N 1/00
C12N 1/12
C12P 19/22
C12N 9/26
C12N 1/00
C12N 1/12
US Classification:
435 41, 435183, 435195, 435200, 435201, 435243, 4352521
Abstract:
Substantially pure glycosidases capable for cleaving selected glycosidic bonds have been described including glycosidases isolated from and recombinant glycosidases. Substrate specificity of isolated enzymes have been identified for GlcNacβ1-x, Galα1-3R, Galα1-6R, Galβ1-3R, Fucα-2R, Fucα1-3R, Fucα1-4R, Manα1-2R, Manα1-3R, Manα1-6R, Manβ1-4R, Xylβ1-2R, Glcβ1-4R, and Galβ1-4R providing improved capability for selectively cleaving a glycosidic linkage in a carbohydrate substrate and for forming modified carbohydrates.
Repair Of Nucleic Acids For Improved Amplification
View pageUS Patent:
7700283, Apr 20, 2010
Filed:
Oct 20, 2005
Appl. No.:
11/255290
Inventors:
Thomas C. Evans - Topsfield MA, US
Barton Slatko - Ipswich MA, US
Lixin Chen - Beverly MA, US
Romaldas Vaisvila - Ipswich MA, US
Chudi Guan - Wenham MA, US
Barton Slatko - Ipswich MA, US
Lixin Chen - Beverly MA, US
Romaldas Vaisvila - Ipswich MA, US
Chudi Guan - Wenham MA, US
Assignee:
New England Biolabs, Inc. - Ipswich MA
International Classification:
C12Q 1/68
US Classification:
435 6, 435 911, 435 912
Abstract:
Methods and compositions are provided for repairing a polynucleotide so that it can be synthesized efficiently with improved fidelity and yield in, for example, an amplification reaction. This involves the use of a reaction mixture that includes a ligase and a cofactor selected from NAD+ or ATP and incubating the polynucleotide with the reaction mixture in the absence of Endonuclease VI. The reaction mixture may further contain an AP endonuclease and a polymerase. These enzymes are optionally selected according to their ability to withstand high temperatures so they can be included in an amplification mixture. The reaction mixture may be used prior to a polynucleotide synthesis reaction in which case enzymes that are not thermophilic may be used. The repair reaction is not time sensitive with respect to seconds, minutes or hours of incubation in the enzyme mixture.
Modified Dna Cleavage Enzymes And Methods For Use
View pageUS Patent:
7851192, Dec 14, 2010
Filed:
Nov 22, 2004
Appl. No.:
10/585964
Inventors:
Chudi Guan - Wenham MA, US
Sanjay Kumar - Ipswich MA, US
Rebecca Kucera - Hamilton MA, US
Sanjay Kumar - Ipswich MA, US
Rebecca Kucera - Hamilton MA, US
Assignee:
New England Biolabs, Inc. - Ipswich MA
International Classification:
C12N 9/16
C12Q 1/44
C12Q 1/68
C07K 14/00
C12N 15/00
C12N 1/21
C12P 21/00
C07H 21/00
C12Q 1/44
C12Q 1/68
C07K 14/00
C12N 15/00
C12N 1/21
C12P 21/00
C07H 21/00
US Classification:
435196, 435 6, 435 19, 435 691, 4353201, 4352523, 530350, 536 232
Abstract:
Compositions and methods are provided that relate to a modified DNA cleaving enzyme having at least 35% amino acid sequence identity with T7 Endo I. The modified enzyme includes two catalytic centers separated by a β-bridge where the β-bridge contains at least one mutation having an effect of altering enzyme cleavage activity compared to the unmodified enzyme. Activities associated with the modified DNA cleaving enzyme that can be modulated in different reaction conditions include at least one of: (a) non-sequence specific nicking activity; (b) cleaving the second strand of a duplex DNA at a preexisting nick site to produce a linear duplex with a single strand overhang; (c) non-sequence specific DNA cleavage; (d) cleaving DNA flanking a mismatch; and (e) cleavage at a cruciform structure in a DNA duplex.
Polynucleotides Encoding Modified Dna Cleavage Enzymes And Host Cells Therefor
View pageUS Patent:
8048664, Nov 1, 2011
Filed:
Nov 18, 2010
Appl. No.:
12/949182
Inventors:
Chudi Guan - Wenham MA, US
Sanjay Kumar - Ipswich MA, US
Rebecca Kucera - Hamilton MA, US
Sanjay Kumar - Ipswich MA, US
Rebecca Kucera - Hamilton MA, US
Assignee:
New England Biolabs, Inc. - Ipswich MA
International Classification:
C12N 15/00
C12N 5/10
C12N 1/21
C07H 21/00
C07K 14/00
C12N 9/16
C12N 9/24
C12P 21/00
C12N 5/10
C12N 1/21
C07H 21/00
C07K 14/00
C12N 9/16
C12N 9/24
C12P 21/00
US Classification:
435196, 4353201, 4352523, 435325, 435 691, 435199, 536 232, 530350
Abstract:
Compositions and methods are provided that relate to a modified DNA cleaving enzyme having at least 35% amino acid sequence identity with T Endo I. The modified enzyme includes two catalytic centers separated by a β-bridge where the β-bridge contains at least one mutation having an effect of altering enzyme cleavage activity compared to the unmodified enzyme. Activities associated with the modified DNA cleaving enzyme that can be modulated in different reaction conditions include at least one of: (a) non-sequence specific nicking activity; (b) cleaving the second strand of a duplex DNA at a preexisting nick site to produce a linear duplex with a single strand overhang; (c) non-sequence specific DNA cleavage; (d) cleaving DNA flanking a mismatch; and (e) cleavage at a cruciform structure in a DNA duplex.
Repair Of Nucleic Acids For Improved Amplification
View pageUS Patent:
8158388, Apr 17, 2012
Filed:
Apr 11, 2006
Appl. No.:
11/401826
Inventors:
Thomas C. Evans - Topsfield MA, US
Lixin Chen - Beverly MA, US
Chudi Guan - Wenham MA, US
Rebecca Kucera - Hamilton MA, US
Barton Slatko - Ipswich MA, US
Romualdas Vaisvila - Ipswich MA, US
Lixin Chen - Beverly MA, US
Chudi Guan - Wenham MA, US
Rebecca Kucera - Hamilton MA, US
Barton Slatko - Ipswich MA, US
Romualdas Vaisvila - Ipswich MA, US
Assignee:
New England Biolabs, Inc. - Ipswich MA
International Classification:
C12P 19/34
US Classification:
435 912
Abstract:
Methods and compositions are provided for repairing a polynucleotide so that it can be copied with improved fidelity and/or yield in, for example, an amplification reaction. This involves the use of a reaction mixture that includes a ligase and a cofactor selected from NAD or ATP and incubating the polynucleotide with the reaction mixture in the absence of Endonuclease VI. The reaction mixture may further contain an AP endonuclease and a polymerase. If used, these enzymes may be selected according to their ability to withstand high temperatures. For example, the reaction mixture may be used prior to a polynucleotide synthesis reaction in which case enzymes that are not thermophilic may be used. The repair reaction is not time sensitive with respect to seconds, minutes or hours of incubation in the enzyme mixture in as much as the repair is effected rapidly and prolonged incubation is not generally adverse.
Repair Of Nucleic Acids For Improved Amplification
View pageUS Patent:
20100173364, Jul 8, 2010
Filed:
Apr 11, 2007
Appl. No.:
12/296827
Inventors:
Thomas C. Evans, JR. - Topsfield MA, US
Barton Slatko - Ipswich MA, US
Lixin Chen - Beverly MA, US
Romaldas Vaisvila - Ipswich MA, US
Chudi Guan - Wenham MA, US
Rebecca Kucera - Hamilton MA, US
Barton Slatko - Ipswich MA, US
Lixin Chen - Beverly MA, US
Romaldas Vaisvila - Ipswich MA, US
Chudi Guan - Wenham MA, US
Rebecca Kucera - Hamilton MA, US
Assignee:
NEW ENGLAND BIOLABS, INC. - Ipswich MA
International Classification:
C12P 19/34
C12N 9/00
C12N 9/10
C12N 9/00
C12N 9/10
US Classification:
435 912, 435 9152, 435183, 435193
Abstract:
Methods and compositions are provided for repairing a polynucleotide so that it can be copied with improved fidelity and/o yield in, for example, an amplification reaction. This involves the use of a reaction mixture that includes a DNA ligase and an effective amount of at least one endonuclease as well as a cofactor selected from NAD or ATP.