Inventors:
Kenneth W. Turteltaub - Livermore CA
John S. Vogel - Union City CA
James S. Felton - Danville CA
Barton L. Gledhill - Alamo CA
Jay C. Davis - Livermore CA
Larry H. Stanker - Livermore CA
Assignee:
Regents of the University of California - Oakland CA
International Classification:
A61K 4300
C01G 2114
A61N 500
Abstract:
Disclosed is a method of quantifying molecules in biological substances, comprising: a. selecting a biological host in which radioisotopes are present in concentrations equal to or less than those in the ambient biosphere, b. preparing a long-lived radioisotope labeled reactive chemical specie, c. administering said chemical specie to said biological host in doses sufficiently low to avoid significant overt damage to the biological system thereof, d. allowing a period of time to elapse sufficient for dissemination and interaction of said chemical specie with said host throughout said biological system of said host, e. isolating a reacted fraction of the biological substance from said host in a manner sufficient to avoid contamination of said substance from extraneous sources, f. converting said fraction of biological substance by suitable means to a material which efficiently produces charged ions in at least one of several possible ion sources without introduction of significant isotopic fractionation, and, g. measuring the radioisotope concentration in said material by means of direct isotopic counting.