Aruna Vashishta

Louisville, KY

Higher Education

University of Louisville Dec 2003 - Feb 2007
Postdoctoral Fellow
University of Louisville Dec 2003 - Feb 2007
Research Associate

Molecular Biology
Western Blotting

United States

Kentucky Spinal Cord Injury Research Center

Mar 2007 to 2000
Research Associate
School of Biotechnology, Jawaharlal Nehru University
New Delhi, Delhi
May 2003 to Nov 2007
Research Associate
University of Louisville
Louisville, KY
Dec 2003 to Feb 2007
Postdoctoral fellow
Doctor of Philosophy, School
New Delhi, Delhi
Aug 1997 to May 2003
Graduate Student Researcher
Doctor of Philosophy, School
New Delhi, Delhi
Aug 1996 to May 1997
Master of Science

Jawaharlal Nehru University
New Delhi, Delhi
Aug 1997 to May 2003
Doctor of Philosophy in Department of Biotechnology
Jawaharlal Nehru University
New Delhi, Delhi
Aug 1995 to May 1997
Masters in Science
Delhi University
New Delhi, Delhi
Aug 1992 to May 1995
Bachelors in Zoology

Technical skills: cDNA and genomic DNA library screening, RNA and DNA isolation, RT-PCR, real time PCR, Cloning, Site directed mutagenesis (SDM), Northern and Southern Blotting, siRNA and shRNA design and transfection, Yeast two-hybrid screening, Gel shift assay, Reporter assay, ChIP assay. ELISA assays, protein expression in bacterial system, protein estimation assays, size exclusion and affinity chromatography, 2-D electrophoresis, Western blotting , Immuoprecipitation, In vitro kinase assay. Transient and stable transfection, Cortical and hippocampal neurons primary culture, DRG culture, Cell lines maintenance, Cell viability and proliferation assays, Invasion assay, General Histology, Immunohistochemistry/ Immunocytochemistry, Inverted and Confocal microscopy. Animal and surgery skills: Collecting materials from mouse pups for genotyping, Genotyping, maintaining colonies of different transgenic mice, Sciatic nerve crush, Intracerebellar injections. Computer Skills: Computing and data analysis: Efficient in using Windows, analyzing data through microsoft excel and sigmaplot, photoshop, coral, Adobe illustrator. Communication and judging Skills: Given seminars and presentations at different symposium and university; good writing skills and also very good organization skills to perform daily duties. Judge posters in scientific meets and also reviewed papers for some scientific journal.