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Alan K Lofquist

from Kirkland, WA
Age ~62
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Also known as:
Alan Keith Lofquist, Alan H Lofquist, Alan K Lofouist, Alan K Loftquist
Phone and address:
9422 130Th Pl, Kirkland, WA 98034
(425) 814-2228
Related to:
Lisa J Johnson, 63Carolyn M Benson, 84Molly WaguespackGerald A Lofquist, 93Mark A Lee, 56Jeanne L Lofquist, 63Thor A Lofquist, 35Paul H Benson, 56Amy L Lofquist, 58Molly W Benson, 56Sarah LeeTyler J Lofquist, 28Henry R Benson, 87Janet R Lofquist, 87Lee Mussig Dana, 69Nicholas D Lofquist, 31Dale Bailey, 45Jeanne L LofguistMark A LeeTamara K Vickaryous, 58Paul H Benson, 56
Connected to:
Chad Lofquist, 39Jeffrey D Lofquist, 61June E Lofquist, 98Marven J Lofquist, 96Scott J Lofquist, 49Steven R Lofquist, 72Thomas Lofquist, 54Jacqueline M Lofranco, 40Denise B Lofrano, 72James F Lofrano, 71

Alan Lofquist Phones & Addresses

  • 9422 130Th Pl, Kirkland, WA 98034 (425) 814-2228
  • 9422 130Th St, Kirkland, WA 98034
  • 9826 122Nd St, Kirkland, WA 98034
  • Carrboro, NC
  • Moscow, ID
  • Saint Paul, MN
  • Kiona, WA
  • 9422 NE 130Th Pl, Kirkland, WA 98034

Skills

Molecular Biology • Immunohistochemistry • Drug Discovery • Cell Culture • Biotechnology • Monoclonal Antibodies • Lifesciences • Cell • Immunology • Technology Transfer • Cell Biology • Clinical Development • ELISA • Assay Development • Antibodies

Industries

Biotechnology

Resumes

Resumes

Alan Lofquist Photo 1

Alan Lofquist

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Location:
Greater Seattle Area
Industry:
Biotechnology
Skills:
Molecular Biology
Immunohistochemistry
Drug Discovery
Cell Culture
Biotechnology
Monoclonal Antibodies
Lifesciences
Cell
Immunology
Technology Transfer
Cell Biology
Clinical Development
ELISA
Assay Development
Antibodies

Publications

Us Patents

Methods For Making Polynucleotide Libraries, Polynucleotide Arrays, And Cell Libraries For High-Throughput Genomics Analysis

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US Patent:
20020094536, Jul 18, 2002
Filed:
Dec 28, 2001
Appl. No.:
10/028970
Inventors:
Alan Lofquist - Seattle WA, US
Robert Finney - Seattle WA, US
David Leung - Seattle WA, US
Assignee:
CELL THERAPEUTICS, INC.
International Classification:
C12Q001/68
C12M001/34
C12N015/00
US Classification:
435/006000, 435/320100, 435/287200
Abstract:
A method for high-throughput, genomics analysis, to identify the therapeutic or diagnostic utility of genes, entails the use of a construct to disrupt a gene or alleles of a gene in cells of interest. Arrays of such cells can be used to monitor such disrupted cells phenotypically in the context, for example, of testing drug candidates. Polynucleotides that comprise part of the disrupted genes can be recovered from such “knockout” cells, by virtue of an origin of replication or a host cell selection marker sequence that is part of the construct. The recovered polynucleotides can be used to identify the disrupted genes or to make homologous recombination vectors, which in turn can be employed to make multi-allele knockout cells.

Methods For Making Polynucleotide Libraries, Polynucleotide Arrays, And Cell Libraries For High-Throughput Genomics Analysis

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US Patent:
20020123065, Sep 5, 2002
Filed:
Mar 15, 2002
Appl. No.:
10/097431
Inventors:
Alan Lofquist - Kirkland WA, US
Robert Finney - Shoreline WA, US
David Leung - Mercer Island WA, US
Assignee:
PanGenex, Inc.
International Classification:
C12Q001/68
G01N033/53
C12M001/34
C12N015/74
US Classification:
435/006000, 435/007100, 435/320100, 435/287200
Abstract:
A method for high-throughput genomics analysis, to identify the therapeutic or diagnostic utility of genes, entails the use of a construct to disrupt a gene or alleles of a gene in cells of interest. Arrays of such cells can be used to monitor such disrupted cells phenotypically in the context, for example, of testing drug candidates. Polynucleotides that comprise part of the disrupted genes can be recovered from such “knockout” cells, by virtue of an origin of replication or a host cell selection marker sequence that is part of the construct. The recovered polynucleotides can be used to identify the disrupted genes or to make homologous recombination vectors, which in turn can be employed to make multi-allele knockout cells.

Methods For Making Polynucleotide Libraries, Polynucleotide Arrays, And Cell Libraries For High-Throughput Genomics Analysis

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US Patent:
20020150945, Oct 17, 2002
Filed:
Jun 13, 2002
Appl. No.:
10/172715
Inventors:
Robert Finney - Shoreline WA, US
David Leung - Mercer Island WA, US
Alan Lofquist - Kirkland WA, US
Assignee:
Cell Therapeutics, Inc.
International Classification:
C12Q001/68
C12M001/34
US Classification:
435/006000, 435/287200
Abstract:
A method for high-throughput genomics analysis, to identify the therapeutic or diagnostic utility of genes, entails the use of a construct to disrupt a gene or alleles of a gene in cells of interest. Arrays of such cells can be used to monitor such disrupted cells phenotypically in the context, for example, of testing drug candidates. Polynucleotides that comprise part of the disrupted genes can be recovered from such “knockout” cells, by virtue of an origin of replication or a host cell selection marker sequence that is part of the construct. The recovered polynucleotides can be used to identify the disrupted genes or to make homologous recombination vectors, which in turn can be employed to make multi-allele knockout cells. Double-stranded RNA molecules designed to target the recovered polynucleotide are used to downregulate the polynucleotide in vitro and in vivo, following determination of a therapeutically effective dosage of the RNAi molecule.

Recombinant Production Of Polyanionic Polymers And Uses Thereof

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US Patent:
20020169125, Nov 14, 2002
Filed:
Mar 20, 2002
Appl. No.:
10/101487
Inventors:
David Leung - Mercer Island WA, US
Philip Bergman - Mountlake Terrace WA, US
Alan Lofquist - Kirkland WA, US
Gregory Pietz - Seattle WA, US
Christopher Tompkins - Bothell WA, US
David Waggoner - Seattle WA, US
Assignee:
CELL THERAPEUTICS, INC.
International Classification:
A61K038/17
A61K038/18
A61K038/19
A61K038/20
A61K038/21
US Classification:
514/012000, 424/085100, 424/085400, 424/085200, 530/350000, 530/351000, 530/399000, 435/069500, 435/069700
Abstract:
A polyanionic polymer can improve the bioactivity and water-solubility properties of a drug to which it is joined. The inventive method provides a monodispersed preparation of a recombinantly-produced polyanionic polymer that can be easily manipulated, such as lengthened. An active moiety may be chemically or recombinantly joined to a polyanionic polymer to increase its biological half-life and/or solubility. The instant invention also provides a method for targeting the delivery of a polyanionic polymer conjugate or fusion protein to a specific cell type or tissue.

Methods For Making Polynucleotide Libraries, Polynucleotide Arrays, And Cell Libraries For High-Throughput Genomics Analysis

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US Patent:
20030143597, Jul 31, 2003
Filed:
Nov 8, 2002
Appl. No.:
10/291235
Inventors:
Robert Finney - Shoreline WA, US
Alan Lofquist - Kirkland WA, US
International Classification:
C12Q001/68
C12M001/34
C12N015/85
C12N005/06
US Classification:
435/006000, 435/325000, 435/287200, 435/455000
Abstract:
A method for high-throughput genomics analysis, to identify the therapeutic or diagnostic utility of genes, entails the use of a construct to disrupt a gene or alleles of a gene in cells of interest. Arrays of such cells can be used to monitor such disrupted cells phenotypically in the context, for example, of testing drug candidates. Polynucleotides that comprise part of the disrupted genes can be recovered from such “knockout” cells, by virtue of an origin of replication or a host cell selection marker sequence that is part of the construct. The recovered polynucleotides can be used to identify the disrupted genes or to make homologous recombination vectors, which in turn can be employed to make multi-allele knockout cells. Double-stranded RNA molecules designed to target the recovered polynucleotide are used to down regulate the polynucleotide in vitro and in vivo, following determination of a therapeutically effective dosage of the RNAi molecule.

Compositions And Methods For Generating Conditional Knockouts

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US Patent:
20040045043, Mar 4, 2004
Filed:
May 19, 2003
Appl. No.:
10/441923
Inventors:
Robert Finney - Shorelin WA, US
Alan Lofquist - Kirkland WA, US
International Classification:
A01K067/00
C12Q001/68
US Classification:
800/008000, 435/006000, 435/069100, 435/325000
Abstract:
The present invention provides vectors and methods for the generation of conditional knockout and knockdown cells and animals. Vectors of the invention may be used to knockout or knockdown an endogenous gene and conditionally regulate the expression of an endogenous or ectopic gene. Accordingly, the invention provides vectors and methods useful for the identification of disease-associated genes, generating animal models of disease, and identifying drug candidates.

Methods For Making Polynucleotide Libraries, Polynucleotide Arrays, And Cell Libraries For High-Throughput Genomics Analysis

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US Patent:
20040137490, Jul 15, 2004
Filed:
Dec 20, 2003
Appl. No.:
10/741084
Inventors:
Robert Finney - Shoreline WA, US
Alan Lofquist - Kirkland WA, US
Assignee:
PanGenex, Inc.
International Classification:
C12Q001/68
C12N015/85
US Classification:
435/006000, 435/455000
Abstract:
A method for high-throughput genomics analysis, to identify the therapeutic or diagnostic utility of genes, entails the use of a construct to disrupt a gene or alleles of a gene in cells of interest. Arrays of such cells can be used to monitor such disrupted cells phenotypically in the context, for example, of testing drug candidates. Polynucleotides that comprise part of the disrupted genes can be recovered from such “knockout” cells, by virtue of an origin of replication or a host cell selection marker sequence that is part of the construct. The recovered polynucleotides can be used to identify the disrupted genes or to make homologous recombination vectors, which in turn can be employed to make multi-allele knockout cells. Double-stranded RNA molecules designed to target the recovered polynucleotide are used to down-regulate the polynucleotide in vitro and in vivo, following determination of a therapeutically effective dosage of the RNAi molecule.

Compositions And Methods For Generating Conditional Knockouts

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US Patent:
20040137572, Jul 15, 2004
Filed:
Dec 20, 2003
Appl. No.:
10/741083
Inventors:
Robert Finney - Shoreline WA, US
Alan Lofquist - Kirkland WA, US
Assignee:
PanGenex, Inc.
International Classification:
C12P021/02
C12N005/06
US Classification:
435/069100, 435/320100, 435/325000
Abstract:
The present invention provides vectors and methods for the generation of conditional knockout and knockdown cells and animals. Vectors of the invention may be used to knockout or knockdown an endogenous gene and conditionally regulate the expression of an endogenous or ectopic gene. Accordingly, the invention provides vectors and methods useful for the identification of disease-associated genes, generating animal models of disease, and identifying drug candidates.
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Alan K Lofquist from Kirkland, WA, age ~62 Get Report