Work:
Pharmacyclics
2014 to 2000
Scientific Manager, Translational Medicine & Clinical Biomarker Program
Life Technologies
Foster City, CA
Jul 2011 to Feb 2013
Staff Scientist, Division of Molecular Medicine, Molecular Diagnostics
Life Technologies
Foster City, CA
Jul 2005 to Jul 2011
Staff Scientist, Genomic Assays-R&D
University of California
San Francisco, CA
Sep 2001 to Jun 2005
Assistant Researcher, Brain Tumor Research Center, Department of Neurological Surgery
University of California
San Francisco, CA
1999 to 2001
Postdoctoral Fellow, Preuss Laboratory for Molecular Neuro
Education:
New York University, School of Medicine
New York, NY
Ph.D. in Department of Pharmacology
National Taiwan University
B.Sc. in Department of Plant Pathology and Entomology
Skills:
PCR, real-time PCR, digital PCR for gene expression, genotyping, copy number, methylation, and allele-specific assays, microarray, sequencing, yeast two-hybrid system, library screening, sub-cloning, nucleic acid extraction from fresh and archived specimens, in vitro transcription and translation, real-time PCR instruments including low- and mid-density microfluidic platforms, robotics for high-throughput liquid handling. Meta-analysis of complex data from high-throughput genomic screening, comparative functional genomics and data mining for molecular profiling, statistic analyses of clinical data. Isolation and explant culture of primary tissues and their induced differentiation, migration, and adhesion, flow cytometry and FACS, immunohistochemistry, in situ hybridization, fluorescence and confocal laser microscopy, digital image analysis, procurement of clinical specimens and histological processing and sectioning. Animal breeding, genotyping of genetic modifications, immunization, general surgical procedure, transcardial perfusion and anatomical dissection, flank and intracranial xenograft, osmotic micropump implantation. IQOQPQ protocol writing and execution, compliance with QSR, ISO, and GLP. Cytokine assays, hybridoma and monoclonal antibody production, enzyme-linked and radio-immunoassays, receptor-ligand binding and enzyme activity assays, neurotransmitter secretion assays. Fractionation and concentration of recombinant and endogenous proteins, affinity chromatography, ion exchange, gel filtration, electrophoresis, Western. Bacterial, yeast, and fungal culture, antagonistic microbe screening, virus inoculation and viral particle purification.
