Yong Wu

VA Medical Center
Bronx, NY
Nov 2001 to Mar 2014
Research Scientist
Neural-pathology Division, Yale Medical School

1997 to 2000
Post Doctor Fellow
University of Alabama at Birmingham
Birmingham, AL
1991 to 1996
Visiting Scholor / Research Associate, Pathology Department

Yale Medical School
New Haven, CT
1997 to 2000
Post-doc Fellow in Neuro-Pathology
China National Academy of Medical Science
1985 to 1988
M.S. in Microbiology and Immunology
Harbin Medical University
1977 to 1982
M.D.

Molecular Biologist with solid gene recombinant skills (Vector construction, gene expression, genomic/cDNA library and Letiviruse expression system construct and package, cell line or clone formation) and biomedical with strong research and development experience Strong expertise with Cells culture, isolation, characterization and line establishment Microarray for gene profiles of muscle cell in different modules and signalling of muscle fibber metabolic (anabolic and catabolic) 96well/386well qPCR for gene expression or profile assay. PBMC sample processing, Cell and serum isolation, storage, QC for PBMC samples and has working experience with microscope and histologic techs DNA/RNA, protein extract and purification. Site direct mutagenesis, DNA sequence SDS-PAGE, Western Blot, Immuno-precipitation, Gel Shift and Foot Print. shRNA or RNAi construct, cloning and knock down the target gene expression. Hands on experience with cell stack/factory format and/or suspension style bioreactor systems Monoclonal antibody purification and conjugation. ELISA, RIA, Flow cytometry. Microscope and histologic techs: IF