Inventors:
- New York NY, US
Andrew Wolfe - New York NY, US
Kamini Sing - New York NY, US
Yi Zhong - New York NY, US
Phillip Drewe - New York NY, US
Assignee:
MEMORIAL SLOAN KETTERING CANCER CENTER - New York NY
International Classification:
G01N 33/50
G01N 33/574
A61K 31/429
A61K 31/357
A61K 31/58
Abstract:
Methods are provided for identifying agents capable of modulating cap-dependent RNA translation by comparing translation efficiency in the presence and absence of the agent in an in-vitro or in-vivo translation system that comprises eIF4A and an mRNA having one or more eIF4A-dependent translation-controlling motifs. The modulation of translation in the presence of the agent indicates the agent as capable of modulating cap-dependent mRNA translation. The method can be used to identify anti-cancer agents and oncogenes that may be responsible for tumorigenesis.
