Inventors:
- Oakland CA, US
- Walnut CA, US
Min Song - Los Angeles CA, US
Libo Zhao - Los Angeles CA, US
Shuang Hou - Los Angeles CA, US
Tom Lee - Redondo Beach CA, US
Daniel Wang - Rowland Heights CA, US
Bo Tao - Chino CA, US
Assignee:
The Regents of the University of California - Oakland CA
CytoLumina Technologies Corp, Inc. - Walnut CA
International Classification:
G01N 1/28
B01L 3/00
Abstract:
A system for isolating preselected cell types from a fluid sample that includes a plurality of cell types includes a cell-capture fluidic chip, and a chip holder configured to receive the cell-capture fluidic chip and to maintain the cell-capture fluidic chip with a substantially fluid-tight seal while in operation. The chip holder is further configured to release the cell-capture fluidic chip to be removed from the chip holder for further processing. The cell-capture fluidic chip includes a substrate, a laser micro-dissection membrane disposed on the substrate, and a channel-defining layer disposed on the laser micro-dissection membrane. The laser micro-dissection membrane has a surface adapted to capture preselected cell types preferentially over other cell types of the plurality of cell types. The channel-defining layer is removable from the laser micro-dissection membrane for further processing of the cell-capture fluidic chip.124