US Patent:
20180372753, Dec 27, 2018
Inventors:
- Oakland CA, US
Tae-Young KIM - Gwangju, KR
Ding WANG - Van Nuys CA, US
Allen KIM - Tujunga CA, US
Edward LAU - Van Nuys CA, US
David A. LIEM - Los Angeles CA, US
Pui Yu LAM - Van Nuys CA, US
Mario DENG - Venice CA, US
Assignee:
The Regents of the University of California - Oakland CA
International Classification:
G01N 33/68
G01N 33/58
G01N 33/60
G01N 30/72
G01N 27/447
G01N 30/88
Abstract:
Disclosed is a method for determining the turnover rate of biomolecules in a subject, which include administering to the subject, HO in an amount sufficient to label biomolecules in the subject with H. Samples are collected from the subject at one or more time points and isotopomers are detected for the labeled biomolecules in the samples. The fractional abundance is determined for the isotopomers of the biomolecules in the samples and the biomolecule turnover rates of the one or more labeled biomolecules is determined based on the fractional abundance of the isotopomers. A computer-implemented method is also disclosed for determining the turnover rate of one or more biomolecules in subject. In certain other embodiments, a system for determining protein turnover rates in a subject is also provided. Also provided in certain embodiments is a computer program product for determining protein turnover rates in a subject.
