Inventors:
Jacqueline K. Barton - San Marino CA, US
Brian Zeglis - Pasadena CA, US
Irvin H. Lau - Pasadena CA, US
Jonathan Hart - San Diego CA, US
Mi Hee Lim - San Diego CA, US
Assignee:
California Institute of Technology - Pasadena CA
International Classification:
C07F 1/00
C12Q 1/68
G01N 33/53
A01N 43/04
US Classification:
544225, 435 6, 435 71, 514 44
Abstract:
In accordance with the present invention there are provided sterically demanding intercalators. These compounds are useful for detection of a base-pair mismatch, such as by measuring fluorescence of complexes formed by the compounds of the invention and nucleic acid duplexes. The compounds are also capable of catalyzing photolytic cleavage of nucleic acids.