Kevin Jing Wu

20230044661, Feb 9, 2023

Dec 17, 2020

17/784133

- Edison NJ, US
Brooke Catalfamo - Chesterfield NJ, US
Michael Zbigniew Kubik - Wallington NJ, US
Subha Bhattacharyya - Metuchen NJ, US
Eric J. Semler - Morganville NJ, US
Yen-Chen Huang - East Brunswick NJ, US
Kevin Wu - Morganville NJ, US
Huy Duc Truong - Middlesex NJ, US

A61L 27/36

Preserved tissue samples contain endogenous viable cells and retain or promote biological activity after being stored at temperatures above freezing for extended periods of time (e.g., from 14 days to 3 years). The preserved tissue samples are implanted in or on a subject and, after rehydration, they retain beneficial biological activity, promote beneficial biological activity, or both. The beneficial biological activity comprises promoting one or more of tissue healing, tissue growth, and tissue generation. Methods for preparing the preserved tissue samples include contacting a recovered tissue sample with one or more protectants, followed by lyopreservation. Suitable protectants include sugars, polyphenols, carotenoids, and combinations thereof. Preferred protectants include glucose, fructose, sucrose, trehalose, dextran, EGCG, and combinations thereof. The recovered tissue sample may be any of several possible issue types. In preferred embodiments, the recovered tissue samples are selected from bone, placental, cartilage and combinations thereof.