Ka A Lee

20200190555, Jun 18, 2020

Feb 27, 2020

16/803542

- St. Paul MN, US
Ka Man Lee - Minneapolis MN, US
Eric R. Peltola - Minneapolis MN, US

C12Q 1/24
B01L 3/00
G01N 1/40
G01N 33/80

Apparatus, system and method for dispensing a particle-laden fluid from a fluid holding and dispensing micro-feature. In some implementations, the apparatus includes: a chamber having one or more surfaces that define a volume to receive fluid containing particulate matter, and an outlet port to dispense at least a portion of the fluid from the chamber. The outlet port may have a normal vector that, when the apparatus is positioned to dispense the fluid, is substantially perpendicular to gravity. The apparatus may be used to measure a number of individual particles from the fluid that flow through the outlet port over a period of them, measure a total volume of the fluid dispensed through the outlet port over the period of time, and calculate a concentration of the particulate matter within the chamber. In some implementations, the particle-laden fluid may be whole blood.

20200033239, Jan 30, 2020

Aug 19, 2019

16/543993

- St. Paul MN, US
Ka Man Lee - Minneapolis MN, US

G01N 1/38
B01L 3/00
G01N 1/30
G01N 15/14
G01N 1/31

The present description provides, in some embodiments, an apparatus for mixing a fluid in a circuit having an inlet channel defining a flow path for a fluid including particulate matter, a first reagent channel in fluid communication with the inlet channel and defining a first reagent flow path for a first reagent, the inlet channel and first reagent channel configured to shear the fluid entering the first reagent channel from the inlet channel at a first junction, a shearing channel in fluid communication with the inlet channel and first reagent channel at the first junction, and a diffusion channel in fluid communication with the shearing channel at a second junction, the sheared fluid collectable into the diffusion channel such that the fluid is compressed at least in part by the first reagent to have a thickness close to a diameter of the particulate matter in the fluid.

20190022651, Jan 24, 2019

Aug 29, 2016

15/755676

- St. Paul MI, US
Elizabeth Palaima - St. Paul MN, US
Pamela Wong - St. Paul MN, US
Daniel R. McPeak - Minneapolis MN, US
Ka Man Lee - Minneapolis MN, US
Eric R. Peltola - Minneapolis MN, US

B01L 3/00
C12Q 1/24
G01N 1/40
G01N 33/80

Apparatus, system and method for dispensing a particle-laden fluid from a fluid holding and dispensing micro-feature and/or multiple lysing channel structures. In some implementations, the apparatus includes: a chamber having one or more surfaces that define a volume to receive fluid containing particulate matter, a soluble surface coating on a portion of the one or more surfaces of the chamber, and an outlet port to dispense at least a portion of the fluid from the chamber. In some implementations, the particle-laden fluid may be whole blood, and the soluble surface coating may include reagents and/or dyes that are diffused into the whole blood received within the chamber to generate signals to visualize various cellular components. In some implementations, the apparatus may also include a second soluble surface coating on portions of surfaces of the multiple lysing channel structures.

20170114386, Apr 27, 2017

Jan 3, 2017

15/397349

- St. Paiul MN, US
Ka Man Lee - Minneapolis MN, US
Eric R. Peltola - Minneapolis MN, US

C12Q 1/24

Apparatus, system and method for dispensing a particle-laden fluid from a fluid holding and dispensing micro-feature. In some implementations, the apparatus includes: a chamber having one or more surfaces that define a volume to receive fluid containing particulate matter, and an outlet port to dispense at least a portion of the fluid from the chamber. The outlet port may have a normal vector that, when the apparatus is positioned to dispense the fluid, is substantially perpendicular to gravity. The apparatus may be used to measure a number of individual particles from the fluid that flow through the outlet port over a period of them, measure a total volume of the fluid dispensed through the outlet port over the period of time, and calculate a concentration of the particulate matter within the chamber. In some implementations, the particle-laden fluid may be whole blood.

20170059459, Mar 2, 2017

May 11, 2016

15/151745

- St. Paul MN, US
Ka Man Lee - Minneapolis MN, US

G01N 1/38
B01L 3/00
G01N 1/30

The present description provides, in some embodiments, an apparatus for mixing a fluid in a circuit having an inlet channel defining a flow path for a fluid including particulate matter, a first reagent channel in fluid communication with the inlet channel and defining a first reagent flow path for a first reagent, the inlet channel and first reagent channel configured to shear the fluid entering the first reagent channel from the inlet channel at a first junction, a shearing channel in fluid communication with the inlet channel and first reagent channel at the first junction, and a diffusion channel in fluid communication with the shearing channel at a second junction, the sheared fluid collectable into the diffusion channel such that the fluid is compressed at least in part by the first reagent to have a thickness close to a diameter of the particulate matter in the fluid.

20170059590, Mar 2, 2017

Aug 27, 2015

14/837524

- St. Paul MN, US
Ka Man Lee - Minneapolis MN, US
Eric R. Peltola - Minneapolis MN, US

G01N 33/80
G01N 1/40

Apparatus, system and method for dispensing a particle-laden fluid from a fluid holding and dispensing micro-feature. In some implementations, the apparatus includes: a chamber having one or more surfaces that define a volume to receive fluid containing particulate matter, and an outlet port to dispense at least a portion of the fluid from the chamber. The outlet port may have a normal vector that, when the apparatus is positioned to dispense the fluid, is substantially perpendicular to gravity. The apparatus may be used to measure a number of individual particles from the fluid that flow through the outlet port over a period of them, measure a total volume of the fluid dispensed through the outlet port over the period of time, and calculate a concentration of the particulate matter within the chamber. In some implementations, the particle-laden fluid may be whole blood.